Result: However, mutation
Q16R resulted in decreased
HBsAg secretion compared to wt M88 (EC/IC ratio: 0.57 versus 1.2) whereas a significant increase in
HBsAg secretion was observed in P38.II (EC/IC ratio: 31 versus 0.5).
Result: Mutations
Q16R,
Q181R and
W182R did not substantially modify the total
HBsAg production pattern in M88 and P38.II plasmids (Figure 4).
Result: Seven
OBI-specific substitutions introducing an arginine residue in
S TMDs were investigated:
Q16R in TMD1,
C85R,
L87R,
L88R, and