Method: The PCR fragment containing the combined 6 core mutations (P5T/S35T/P79Q/E83D/S87G/Q177K) were amplified from pCMVHBV-GYF-L97I and the PCR product was used as primers to mutate pCMVHBV-WT by Q5 mutagenesis, giving rise to plasmid pCMVHBV-WT-6Cmut.
Result: Furthermore, introducing I97L mutation into WT did not enhance viral DNA replication, but changing WT core to other six mutations together (P5T, S35T, P79Q, E83D, S87G, and Q177K) signif
Core I97L mutation in conjunction with P79Q is associated with persistent low HBV DNA and HBs antigen clearance in patients with chronic hepatitis B.
PMID: 27998820
2017
Clinical microbiology and infection
Abstract: CONCLUSIONS: In patients with CHB, measurement of I97L and additional mutation P79Q would be useful for predicting persistent low HBV DNA, normal ALT, and HBsAg clearance.
Abstract: Cumulative incidences of persistent low HBV DNA and HBsAg clearance were significantly higher in patients with I97L than in those with wild-type I97 (p = 0.003 and p = 0.016, respectively), and even higher in those with P79Q.
Abstract: Subsequently, 82 patients with CHB were evaluated for the I97L mutation and the additional mutation P79Q.
Abstract: We compared cumulative incidences of persistent low HBV DNA an