Precore/core mutations of hepatitis B virus genotype D arising in different states of infection.
PMID: 35415256
2022
Clinical and experimental hepatology
Introduction: In a study conducted in the Korean population, the five HBcAg mutations P5H/L/T, E83D, I97F/L, L100I, and Q182K/Stop were significantly more frequent in subjects with chronic hepatitis and cirrhosis.
Conformational Plasticity of Hepatitis B Core Protein Spikes Promotes Peptide Binding Independent of the Secretion Phenotype.
1Abstract: The peptide ""GSLLGRMKGA"" binds weakly to hepatitis B core protein capsids and mutant capsids with a premature (F97L) or low-secretion phenotype (L60V and P5T)."
Abstract: With electron cryo microscopy, we provide novel structures for L60V and P5T and demonstrate that binding occurs at the tips of the spikes at the dimer interface, splaying the helices apart independent of the secretion phenotype.
Introduction: Some naturally occurring mutations at this site is also implicated with either low-level secretion (P5T-HBc and L60V-HBc) or premature (F/I/97 L-
Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97.
Abstract: Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L).
Introduction: Naturally occurring point mutations like HBc-F/I97L with an immature secretion phenotype or point mutations with a low secretion phenotype, such as HBc-L60V and HBc-P5T, are located in the area surrounding the pocket.
Method: For all other variants, the final concentration of TX100 was 2 mM and the HBc-monomer concentrations were 700 microM for HBc-P5T and HBc-
Naturally occurring core protein mutations compensate for the reduced replication fitness of a lamivudine-resistant HBV isolate.
Abstract: Further mutagenesis study revealed that the P5T mutation of core protein plays an important role in the enhanced viral replication through increasing the levels of capsid formation and pregenomic RNA encapsidation.
Introduction: However, the mechanism of P5T-associated liver disease progression remains unclear.
Introduction: Moreover, it has also been suggested that P5T/A mutation is an independent factor for acute-on-chronic liver failure (ACLF).
Introduction: Our study revealed that the mutant core, predominantly P5T, boosts the levels of HBV capsid format
Persistence of Hepatitis B Virus DNA and the Tempos between Virion Secretion and Genome Maturation in a Mouse Model.
3Introduction: Interestingly, while a single HBc mutation L60V or P5T displayed a low-level secretion phenotype, a single mutation P130T led to a ""hypermaturation phenotype"" characterized by an increased abundance of both intracellular and extracellular fully mature full-length RC DNA."
Introduction: Both P130T and P5T could compensate for or rescue the immature secretion phenotype of mutant I97L.
Introduction: In addition to this HBc 97L mutation, HBc P130T and P5T mutations were found frequently in patients.
The Correlation Between Hepatitis B Virus Precore/Core Mutations and the Progression of Severe Liver Disease.
PMID: 30406036
2018
Frontiers in cellular and infection microbiology
Introduction: Furthermore, five HBcAg mutations identified in a Korean population, P5H/L/T, E83D, I97F/L, L100I, and Q182K/Stop, were shown to be significantly enriched in HCC patients compared with patients at earlier disease stages, including those with cirrhosis and chronic hepatitis (Kim et al.,).
Introduction: Similarly, a study of an Indian population showed that HBcAg residue 5 mutations P5H/L/T or P5R were found more frequently in HCC pati
Upregulation of endoplasmic reticulum stress and reactive oxygen species by naturally occurring mutations in hepatitis B virus core antigen.
PMID: 25828947
2015
The Journal of general virology
Abstract: Here, we found that the naturally occurring mutations P5T/H/L of the HBV core antigen induced ER stress.
Novel point and combo-mutations in the genome of hepatitis B virus-genotype D: characterization and impact on liver disease progression to hepatocellular carcinoma.
8Discussion: Interestingly, the most frequently observed naturally occurring core protein mutation found in CHB and also in HCC patients I97L or F97L which is associated with ""immature virion secretion"" was completely absent in Indian patients with HBV-genotype D while among the ""low secretion core variants"" such as P5T/A/S, a new variant P5R was noted with high frequency in HBV-genotype D related HCC in previous Indian study."
Naturally occurring precore/core region mutations of hepatitis B virus genotype C related to hepatocellular carcinoma.
Abstract: Six (preC-W28*, C-P5H/L/T, C-E83D, C-I97F/L, C-L100I and C-Q182K/*) and seven types (preC-W28*, preC-G29D, C-D32N/H, C-E43K, C-P50A/H/Y, C- PMID: 19327810
2009
Virology
Discussion: In contrast, a P5T mutation impairs virion secretion when present alone and restores wild-type secretion phenotype when combined with the I97L mutation.
HBV mutation literature information.
PMID: 
2022
Clinical and experimental hepatology
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