Abstract: The highest frequency of mutations S101F (62.2%), A102V/R/G/I (56.25%), M103L/A (68.75%)were found in HCC, followed in LC and CH patients as 57.1%, 42.8%, 28.52% 16%, 15.2% and 18.4% respectively.

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Result: Sequencing and data analysis showed the highest frequency of S101F (62.2%), A102V/R/G/I (56.25%), and M103L/A (68.75%) mutation in HCC and 57.1%, 42.8%, and 28.52% respectively in LC as compared to 16%, 15.2%, and 18.4% respectively in