Introduction: Confocal microscopy revealed that the L77R mutant HBsAg are largely accumulated within the ER and Golgi.
Introduction: In our earlier studies, a naturally occurring S gene mutation L77R at the large loop CYL-I resulted in more than tenfold reduced HBV virion secretion, and 2.8-fold reduced HBsAg in the medium of transfected HuH-7 cells.
Expansion of viral variants associated with immune escape and impaired virion secretion in patients with HBV reactivation after resolved infection.
Abstract: Furthermore, reactivated HBV in 11 of 16 (69%) non-HSCT cases possessed substitutions associated with impaired virion secretion, including E2G, L77R, L98V, T118K, and Q129H in the S region, and M1I/V in the PreS2 region.
Result: Amino acid substitutions of sE2G (n = 3), sL77R (n = 1), and sL98V (n = 1) in the small S protein that were associated with impaired virion secretion were also the pr
Surface gene variants of hepatitis B Virus in Saudi Patients.
PMID: 26997220
2016
Saudi journal of gastroenterology
Result: These changes include an overall of 11 unique sites that locate in the three different fragments of S proteins; D16E, T40P, F56L, Q75H, L77R, Q107K, and W111Sc in pre-S1, S135F in pre-S2, and F183S, C239Y, and Y369H in S.
Reduced secretion of virions and hepatitis B virus (HBV) surface antigen of a naturally occurring HBV variant correlates with the accumulation of the small S envelope protein in the endoplasmic reticulum and Golgi apparatus.
Abstract: Despite the accumulation of L77R HBsAg in ER-Golgi of transfected Huh7 cells, we detected no increase in Grp78 mRNA and proteins, which are common markers for ER stress response.
Abstract: In addition, the 2.8-fold reduction of the extracellular HBV surface antigen (HBsAg) of mutant L77R from transfected Huh7 cells appeared to be correlated with a 1.7-fold reduction of intracellular HBsAg, as measured by enzyme-linked immunosorbent assay (ELISA).
Abstract: In contrast to HBsAg, the secretion of HBeAg was normal in L77R-transfected cells.
HBV mutation literature information.
PMID: 
2022
PloS one
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