Result: Exceptionally, 13 S mutations presented the higher distributions (p<0.1) in the HCC group: F20S (8.2% vs 1%, p = 0.015), D33G (4.1% vs 0%, p = 0.039), (T47A/E/V/K (18.4% vs 7.1%, p = 0.025), R79H (6.1% vs 0%, p = 0.007), L88P (4.1% vs 0%, p = 0.039), P120S/T (on the MHR region, 16.3% vs 6.6%, p = 0.042), G145R (6.1% vs 1%, p = 0.055), S174N (6.1% vs 0.5%, p = 0.026), V190A (6.1% vs 0.5%, p = 0.026), P203R (8.2% vs 2%, p = 0.052), Y206H/F/C (6.1% vs 1.5%, p = 0.094),