Precore/core mutations of hepatitis B virus genotype D arising in different states of infection.
PMID: 35415256
2022
Clinical and experimental hepatology
Introduction: In a study conducted in the Korean population, the five HBcAg mutations P5H/L/T, E83D, I97F/L, L100I, and Q182K/Stop were significantly more frequent in subjects with chronic hepatitis and cirrhosis.
rt269I Type of Hepatitis B Virus (HBV) Polymerase versus rt269L Is More Prone to Mutations within HBV Genome in Chronic Patients Infected with Genotype C2: Evidence from Analysis of Full HBV Genotype C2 Genome.
Abstract: We also found that there are a total of 19 signature single nucleotide polymorphisms (SNPs), of which 2 and 17 nonsynonymous mutation types were specific to rt269L and rt269I, respectively: Of these, most are HBeAg negative infections (preC-W28*, X-V5M and V131I), lowered HBV DNA or virion production (C-I97F/L, rtM204I/V) or preexisting nucleot(s)ide analog resistance (NAr) (rtN139K/H, rtM204I/V and
Amino Acid Polymorphism in Hepatitis B Virus Associated With Functional Cure.
PMID: 34352407
2021
Cellular and molecular gastroenterology and hepatology
Abstract: CONCLUSIONS: The I97L substitution reduces the level of cccDNA through the generation of immature virions with single-stranded genomes.
Abstract: HBV-I97L exhibited lower infectivity than HBV-I97wt in both infection systems with reporter HBV and cell culture-generated HBV.
Abstract: In this study, we attempted to identify the point at which I97L affects the hepatitis B virus (HBV) life cycle and to elucidate the underlying mechanisms governing the stabilization of hepatitis.
Abstract: METHODS: To confirm the clinical features of I97L, we used a cohort of hepatitis B e antigen-negative patients with PMID: 34834922
2021
Viruses
Introduction: Naturally occurring point mutations like HBc-F/I97L with an immature secretion phenotype or point mutations with a low secretion phenotype, such as HBc-L60V and HBc-P5T, are located in the area surrounding the pocket.
A nuanced role of the small loop of hepatitis B virus small envelope protein in virion morphogenesis and secretion.
8Discussion: Naturally occurring core mutants I97L (isoleucine to leucine) or F97L (phenylalanine to leucine), exhibited a so-called ""immature secretion"" phenotype, which allows excessive secretion of virions containing an immature genome."
Discussion: A pre-S1 envelope mutation A119F, changing an alanine (A) to a phenylalanine (F), can erase the immature secretion phenotype of the mutant I97L and successfully restore the wild-type-like selective export of the mature genome.
Naturally occurring core protein mutations compensate for the reduced replication fitness of a lamivudine-resistant HBV isolate.
Method: The core gene point mutations (P5T, S35T, S87G, I97L, Q177K) and the reversion mutations (T5P, T35S, Q79P, D83E, G87S, L97I, K177Q) were individually introduced into pCMVHBV-WT and pCMVHBV-GYF, respectively, by using QuikChange Site-directed Mutagenesis Kit (Stratagene) or Q5 Site-directed Mutagenesis Kit (NEB).
Result: Among those, I97L has been reported to be associated with the increased HBV DNA replication in Huh7 cells, we thus first examined the potential role of I97L
Persistence of Hepatitis B Virus DNA and the Tempos between Virion Secretion and Genome Maturation in a Mouse Model.
Abstract: Although mutant P130T also displayed a hypermaturation phenotype in vivo, it cannot efficiently rescue the immature virion secretion of mutant I97L.
Abstract: Although viral DNA of mutant I97L with immature genome is less persistent than wild-type HBV in time course experiments, viral DNA of mutant P130T with genome hypermaturation, surprisingly, is more persistent.
Abstract: Here, we demonstrated that mutant I97L can secrete immature genome in mice.
Abstract: However, a frequent naturally occurring HBc variant, I97L, changing from an isoleucine to a leucine at amino acid 97, exhibited an immature secretion phenotype in culture, which preferentially secretes virions containing immature genom
Early changes in quasispecies variant after antiviral therapy for chronic hepatitis B.
Abstract: Furthermore, several mutation patterns, such as cI97L and cP130T showed alterations in the secondary structure and predicted antigenicity of HBV protein.
The Correlation Between Hepatitis B Virus Precore/Core Mutations and the Progression of Severe Liver Disease.
PMID: 30406036
2018
Frontiers in cellular and infection microbiology
Introduction: Furthermore, five HBcAg mutations identified in a Korean population, P5H/L/T, E83D, I97F/L, L100I, and Q182K/Stop, were shown to be significantly enriched in HCC patients compared with patients at earlier disease stages, including those with cirrhosis and chronic hepatitis (Kim et al.,).
Core I97L mutation in conjunction with P79Q is associated with persistent low HBV DNA and HBs antigen clearance in patients with chronic hepatitis B.
PMID: 27998820
2017
Clinical microbiology and infection
Abstract: CONCLUSIONS: In patients with CHB, measurement of I97L and additional mutation P79Q would be useful for predicting persistent low HBV DNA, normal ALT, and HBsAg clearance.
Abstract: Cumulative incidences of persistent low HBV DNA and HBsAg clearance were significantly higher in patients with I97L than in those with wild-type I97 (p = 0.003 and p = 0.016, respectively), and even higher in those with P79Q.
Abstract: RESULTS: Incidence of Core mutation I97L differed significantly among groups: A, 30% (3/10); B, 36.4% (4/11); C, 83.3% (10/12) (p = 0.021).
HBV mutation literature information.
PMID: 
2022
Clinical and experimental hepatology
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