Abstract: METHODS: Primary human hepatocytes were infected with HBV using genomic plasmids carrying the basic core promoter mutation A1762T/G1764A and/or the precore mutation G1896A and treated with IFN-gamma and IFN-alpha.
Combining the HBcrAg decline and HBV mutations predicts spontaneous HBeAg seroconversion in chronic hepatitis B patients during the immune clearance phase.
Abstract: Baseline A1574T, G1862A, G1896A, and C1913G mutations and HBcrAg levels with a sharp decrease at Week 28 were associated with spontaneous HBeAg seroconversion.
Abstract: The mutation frequencies of A1574T (51.11% vs. 18.18%, p = 0.001), G1862A (30.00% vs. 13.03%, p = 0.001), G1896A (27.22% vs. 5.45%, p = 0.001), and C1913G (32.78% vs. 12.73%, p = 0.001) in Group A were significantly higher than Group B.
Prevalence, genotype distribution and mutations of hepatitis B virus and the associated risk factors among pregnant women residing in the northern shores of Persian Gulf, Iran.
Result: Moreover, a G to A substitution at nucleotide position 1896 (G1896A) was detected in the pre-C region of one sample (HB55) (S2 Fig), this sample was negative for HBeAg.
Discussion: Notably, the sample with G1896A mutation was HBeAg negative.
Discussion: Of these, the pre-C mutation (G1896A) eliminates HBeAg expression at the translational level, whereas BCP mutations (G1764A) decrease HBeAg expression at the transcriptional level.
Discussion: The G1896A mutation causes the conversion of TGG TAG (Tr
Reactivation of Occult Hepatitis B Virus Infection During Long-Term Entecavir Antiviral Therapy.
Abstract: Genetic sequencing of HBV showed the mutants of S143T, D144G, and G145R in the S gene region, and the mutant of site 1896 in the pre-Core region coexisted with the wild type (G1896A/G).
Result: Mutation analysis of this patient's HBV showed there were three mutants (S143T, D144G, and G145R) in the S gene region, and the mutant of site 1896 in the pre-Core region coexisted with the wild type (G1896A/G), but no mutation in the pre-S1, pre-S2, and
Precore/core mutations of hepatitis B virus genotype D arising in different states of infection.
PMID: 35415256
2022
Clinical and experimental hepatology
Abstract: Results: The stop codon of W28*(G1896A) was determined as the most prevalent mutation (55%) of the precore region.
Discussion: The result of our study showed that a precore point mutation, G1896A, that converts the tryptophan to a stop codon (W28*), was the most common variation in the patients, as detected in chronic HBV in Asia and the Mediterranean region.
Discussion: reported that 31.8% (14/44) of patients had mutations in the precore region (G1896A).
Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection.
Abstract: In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera.
Method: To produce HBeAg of various genotypes, a serial of HBV 1.3-fold or CMV-driven 1.1-fold genome plasmids with genotypes A-I (detailed information was shown in supplemental Table 1) or with precore G1896A mutation were transfected into HepG2 cells or Huh7 cells using X-tremeGENE HP DNA Transfection Reagent (Roche).
Result: As HBV genotype G permanently harbours C1817T and G1896A nonsense mutations in the precor
Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China.
Discussion: G1896A is another typical mutation in the pre-C region, which generates a stop codon at the 28th amino acid position of the HBeAg sequence, resulting in the inhibition of protein synthesis.
Transcriptome analysis of hepatoma cells transfected with Basal Core Promoter (BCP) and Pre-Core (PC) mutant hepatitis B virus full genome construct.
PMID: 33595430
2021
The Journal of general virology
Abstract: Infections with Basal Core Promoter (BCP) (A1762T/G1764A) and Pre-Core (PC) (G1896A) hepatitis B virus HBeAg mutants are associated with severe liver injury.
Hepatitis B virus precore/core region mutations and genotypes among hepatitis B virus chronic carriers in South-Eastern, Nigeria.
PMID: 33708042
2021
International journal of health sciences
Introduction: The precore stop codon mutation (G1896A) is resistant to IFN treatment.
Introduction: The HBV precore G1896A mutation is known to be associated with HBV reactivation.
Discussion: The
Discussion: The G1896A mutation has previously been reported among HIV/HBV co-infected Nigerians in a study done in Jos, North-central, Nigeria as 57%.
Discussion: The G1896A mutation is a nonsense mutation occurring in the HBV precore region leading to a G-to-A shift, which causes a conversion of codon 28 from TGG (tryptophan) to a premature stop codon TAG, and subsequent termination of the expression of HBeAg.
The Occurrence of rtA194T Mutant After Long-Term Lamivudine Monotherapy Remains Sensitive to Tenofovir Disoproxil Fumarate: A Case Report.
Discussion: However, G1896A or A1762T/G1764A mutations were not found in this patient with genotype C HBV by DNA sequencing, while A1727T, C1730G and C1799G mutations were found in BCP region, which were reported to be associated significantly with cirrhosis.
HBV mutation literature information.
PMID: 
2022
Hepatology research
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