Abstract: I127T, V131I, and F132Y/I/R mutations showed a significant increasing trend associated with the disease progression to HCC.
Abstract: One double mutation (K130M+V131I) and two triple mutations (I127T+K130M+V131L and K130M+V131I+F132Y) were observed, with significant rising prevalence through progressive clinical phases of liver disease to HCC.
Result: Both AC and LC gr
HBx mutations promote hepatoma cell migration through the Wnt/beta-catenin signaling pathway.
Introduction: 127 (isoleucine to asparagine/serine/threonine), 130 (lysine to methionine), 131 (valine to isoleucine), and 132 (phenylalanine to tyrosine), which might affect the biological activity of HBx.18, 19 However, the potential role of HBx Combo mutations in hepatocarcinogenesis is largely unknown.
Discussion: substitutions (I127N/K130M/V131I/F132Y) that may lead to aberrant biological activity of HBx.
Variability in the precore and core promoter regions of HBV strains in Morocco: characterization and impact on liver disease progression.
Discussion: Moreover, T1768A result in aa changes at HBx codon 132 (F132Y), that have been showed to play a synergetic role with K130M and V131I introduced by A1762T/G1764A for leading to carcinogenesis.
Hepatitis B virus core promoter mutations contribute to hepatocarcinogenesis by deregulating SKP2 and its target, p21.
Method: In addition, a combination mutant of I127N/K130M/V131/I/F132Y (abbreviated as Combo mutant) was generated.
Method: To generate HBx mutants that correspond to mutations in the basal core promoter region, site-directed mutagenesis was performed at amino acid positions I127N (T1753A), K130M/V131I (A1762T/G1764A) (abbreviated as TA mutant) and F132Y (T1768A) using a Quikchange kit (Stratagene) according to the manufacturer's protocol.
Discussion: Our results in HepG2 and Huh7cells ind
Genetic dissection of naturally occurring basal core promoter mutations of hepatitis B virus reveals a silent phenotype in the overlapping X gene.
PMID: 19439550
2009
The Journal of general virology
Abstract: Due to a compact viral genome organization, BCP1 and BCP2 mutations result in amino acids changes in the overlapping X gene: K130M/V131I and F132Y, respectively.
"Biological impacts of ""hot-spot"" mutations of hepatitis B virus X proteins are genotype B and C differentiated."
PMID: 16094714
2005
World journal of gastroenterology
1Abstract: METHODS: Five types of ""hot-spot"" mutations of genotype B or C HBV X genes, which sequentially lead to the amino acid substitutions of HBx as I127T, F132Y, K130M+V131I, I127T+K130M+V131I, or K130M+V131I+F132Y, respectively, were generated by means of site-directed mutagenesis."
Abstract: RESULTS: As compared to standard genotype B HBx, mutants of I127T and I127T+K130M+V131I showed high
Mutations in the transcriptional regulatory region of the precore and core/pregenome of a hepatitis B virus with defective HBeAg production.
Abstract: The deduced amino acid substitutions were 28 Arg--Gln, 94 His--Tyr, 131 Val--Ile and 132 Phe--Tyr of HBx and 715 Met--Val and 789 Asp--Asn of pol.
HBV mutation literature information.
PMID: 
2017
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