Result: Three HBsAg cDNAs, namely W1S, W3S (subtype adr) and adr4 (subtype adr, genotype C), were cloned into a mammalian expression vector (pEBV His) and expressed by transient transfection in HEK293-T cells.

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Result: Thus, we speculated that the impaired reactivity (antigenicity) of the W3S HBsAg might have been due to natural mutations in its amino acid sequence that occurred at different positions compared to those in the adr4 and W1S HBsAgs (Fig 1A).

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Result: To confirm the effect of amino acid mutations within the

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