Amino acid substitutions Q129N and T131N/M133T in hepatitis B surface antigen (HBsAg) interfere with the immunogenicity of the corresponding HBsAg or viral replication ability.
Abstract: Expression plasmids of vtHBsAg with aa substitutions Q129 L, T123 N, Q129 N and T131 N/M133 T were constructed.
Immune-escape mutations and stop-codons in HBsAg develop in a large proportion of patients with chronic HBV infection exposed to anti-HBV drugs in Europe.
Discussion: Antigenic index (Ai) values of four or more amino acids around amino acid position 123 were altered by a magnitude of -0.4 to +0.2 or more by the T123A or T123N mutations, respectively.
Discussion: On the other hand, when threonine at position 123 (T123) was substituted with asparagine (T123N), the reactivity of HBsAg in the supernatant of transfected cells was significantly reduced, but HBsAg retained some degree of its reactivity with the cell lysate or with other antibodies.
HBV genotypes and drug resistance mutations in antiretroviral treatment-naive and treatment-experienced HBV-HIV-coinfected patients.
Method: The sequences were examined for known vaccine escape mutations (sG145R/A, sP142S, sI/T126A/N/I/S, sQ129H/R, sM133L, sD144A/E, sP120S/E, sK141E, sP134I, and sT116N), immunoprophylaxis escape mutations (
"Localization of immunodominant epitopes within the ""a"" determinant of hepatitis B surface antigen using monoclonal antibodies."
Abstract: In the present study, 1.3-fold-overlength HBV genomes containing the sT123N substitution were digested from the pHBV1.3-sT123N construct and subcloned into the pAAV vector to generate pAAV1.3-sT123N for hydrodynamic injection (HI) in mice.
Abstract: The results demonstrated that sT123N substitution impaired virion secretion, resulting in intracellular retention of HBcAg.
Abstract: We have previously showed that sT123N substitution could generate additional N-glycosylated forms of HBsAg.
"Construction and expression of hepatitis B surface antigen escape variants within the ""a"" determinant by site directed mutagenesis."
1Abstract: RESULTS: Ten HBsAg mutants having single mutation within the ""a"" determinant including P120E, T123N, Q129H, M133L, K141E, P142S, D144A, G145R, N146S and C147S together with a wt form were successfully constructed and expressed in CHO cells."
A multicentre molecular analysis of hepatitis B and blood-borne virus coinfections in Viet Nam.
Result: Mutations associated with immune escape were identified in 23.5% (n = 45/187) samples and included the following residues Y100C (n = 6), T118K (n = 1), P120L/Q/S/T (n = 10), T123A/N (n = 2), I126N/S (n = 8), P127S (n = 2), Q129R (n = 2), G130D/N/R (n = 1), T131I (n = 4), M133L/T (n = 18), G144E (n = 1) and G145A/R (n = 4).
Characteristics of occult hepatitis B virus infection in the Solomon Islands.
PMID: 21455562
2011
International journal of molecular medicine
Abstract: Most of the HBV-DNA-positive individuals were infected with wild-type HBV, and only 3 strains demonstrated specific amino acid substitutions (P121X, T123N, C138S, P142S and D144E) in the alpha determinant region.
HBV mutation literature information.
PMID: 
2018
Virus research
Browser Board
Co-occurred Entities
Filtrator
ViMRT