Method: The core gene point mutations (P5T, S35T, S87G, I97L, Q177K) and the reversion mutations (T5P, T35S, Q79P, D83E, G87S, L97I, K177Q) were individually introduced into pCMVHBV-WT and pCMVHBV-GYF, respectively, by using QuikChange Site-directed Mutagenesis Kit (Stratagene) or Q5 Site-directed Mutagenesis Kit (NEB).
Method: The PCR fragment containing the combined 6 core mutations (P5T/S35T/P79Q/ PMID: 25333524
2014
PloS one
Result: Overall three mutations in Th epitope (T12S, S35T, T67N) and one in CTL epitope (T147C) of core showed significant association with HCC (p<0.05) (Table 3).
Table: S35T
Discussion: Koschel et al showed that deletion at codon 12 is defective in virion secretion while other two mutations S35T, T67N might be able to evade the host immune response for their persistence at low levels.
Discussion: While three mutations in Th epitopes (T12S, S35T, T67N), T147C in CTL e
HBV mutation literature information.
PMID: 
2019
Antiviral research
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