Result: N146S showed a comparable binding to both antibodies.
Result: In contrast, the polyclonal anti-HBsAg antibody signal was severely reduced in several mutants within the 2nd loop of 'a' determinant, such as K141I, P142L/S, D144A/E, G145K/R, N146S and T148I.
Occult HBV infection in Chinese blood donors: role of N-glycosylation mutations and amino acid substitutions in S protein transmembrane domains.
Result: Introduction of the N146D/S/Y mutations in plasmid M88 and P38.II by SDM did not significantly alter the EC and IC HBsAg production pattern, although a slight reduction in HBsAg secretion was observed for P38.II mutants, as reflected by the decreased HBsAg EC/IC ratio compared to that of wt HBsAg (5-8 versus 17) (Figure 3).
Result: Mutants N146D/S/Y showed an expected non-glycosylated profile.
Result: Particularly, mutations removing the N146 glycosylation site (N146D/S/Y) were found in 4.4% (2/45) and 6% (3/50) of OBI genotype B (OBIB) and genotype C (OBIC) sequences, respectively.
Impact of immune escape mutations and N-linked glycosylation on the secretion of hepatitis B virus virions and subviral particles: Role of the small envelope protein.
Introduction: Abolishing N-linked glycosylation in the S domain by either N146Q or N146S mutation prevented the secretion of virions but not subviral particles (HBsAg).
Introduction: Our previous study revealed that the M133T mutation, which creates a novel glycosylation site at N131 (131NST133), could rescue virion secretion of the N146Q and N146S mutants.
Result: Mutations that abolish N-linked glycosylation (N146Q and N146S) or create an extra glycosylation site (M133T) did not markedly affect HBsAg secretion.
Discussion: For the glycosylation mutants, both N14
Immune-escape mutations and stop-codons in HBsAg develop in a large proportion of patients with chronic HBV infection exposed to anti-HBV drugs in Europe.
1Abstract: RESULTS: Ten HBsAg mutants having single mutation within the ""a"" determinant including P120E, T123N, Q129H, M133L, K141E, P142S, D144A, G145R, N146S and C147S together with a wt form were successfully constructed and expressed in CHO cells."
Detection of a hepatitis B surface antigen variant emerging in a patient with chronic lymphocytic leukaemia treated with fludarabine.
1Abstract: These changes involved 11 positions inside and 5 outside of the historical first and second loops of the ""a"" determinant, and included the following: Q101K, T115A, K122N, T123A, T126N, Q129N, G130R, T131I, M133T, F134L, C138Y, K141E, P142S, G145R, N146S, and C147F/R."
HBV mutation literature information.
PMID: 
2020
PloS one
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