Randomized comparison of tenofovir disoproxil fumarate vs emtricitabine and tenofovir disoproxil fumarate in patients with lamivudine-resistant chronic hepatitis B.
Abstract: Patients were hepatitis B e antigen (HBeAg)-positive or HBeAg-negative, with levels of HBV DNA >=3 log10 IU/mL and lamivudine resistance mutations (HBV polymerase or reverse transcriptase amino acid substitutions rtM204I/V +- rtL180M by INNO-LiPA Multi-DR v3; Innogenetics, Inc, Alpharetta, GA).
Rapid detection of hepatitis B virus variants associated with lamivudine and adefovir resistance by multiplex ligation-dependent probe amplification combined with real-time PCR.
PMID: 24478474
2014
Journal of clinical microbiology
Abstract: Based on the results of MLP-RT-PCR, the mutations rtM204V, rtM204I, rtA181T, rtA181V, and rtN236T were 95.7% (111/116 patients), 98.3% (114/116 patients), 99.1% (115/116 patients), 98.3% (114/116 patients), and 99.1% (115/116 patients) concordant, respectively, with those of direct sequencing.
Abstract: For this study, a multiplex ligation-dependent probe real-time PCR (MLP-RT-PCR) method was developed to simultaneously detect lamivudine (LAM)- and adefovir (ADV)-resistant HBV mutants (those with the mutations rtM204V/I, rt
Drug-resistance associated mutations in polymerase (p) gene of hepatitis B virus isolated from malaysian HBV carriers.
Abstract: A number of significant drug resistant mutations were found in five patients including S202I, N236T, M250L, L180M/V, M204I, A181T, T184G, M250V, and V173L.
Abstract: Of these, L180M/V and M204I were most frequently detected (80%) and associated with lamivudine in combination with emtricitabine and telbivudine drug resistance.
Discussion: Adefovir resistance was caused by N236T and /or A181V amino acid substitution, whereas entecavir resistance resulted from HBV reverse transcri
rtM204Q may serve as a novel lamivudine-resistance-associated mutation of hepatitis B virus.
Result: By contrast, rtM204I/rtM204V was detected in 2,502 patients with an incidence of 25.5% (2,502/9,830).
Result: Clonal sequencing (35 clones) of the sample at months 24 (Sa3) showed that rtM204Q, rtA181T, rtM204I, rtA181T+M204Q and wild-type strains occupied 14%, 14%, 3%, 0%, and 69%, respectively.
Result: Clonal sequencing (37 clones) of the sample at this point (Sa2) showed coexistence of rtM204Q (30%), rt
Establishment of real time allele specific locked nucleic acid quantitative PCR for detection of HBV YIDD (ATT) mutation and evaluation of its application.
Figure: Typical chromatograms of the cloning sequencing of HBV rtM204 and rtM204I.
Discussion: In this study, we developed a new assay for the detection of the variant rtM204I with high-sensitivity.
Discussion: It is known that rtM204I is one of the most important and common resistance mutation sites, which can not only cause the selection of LMV and LdT resistance, but also lead to ETV resistance.
Discussion: Therefore, establishing a simple, rapid, reliable and highly sensitive assay to detect the rt PMID: 24632784
2014
PloS one
Result: The two others, LDA173 and LDA339, displayed the lamivudine resistance triple mutation rtV173L, rtL180M, rtM204V/I which causes the concomitant amino acid substitutions E164D/G and I195M in the small S protein (Table 3).
Novel natural mutations in the hepatitis B virus reverse transcriptase domain associated with hepatocellular carcinoma.
Result: It is worth noting that we did not observe any of the common NA-related resistance mutations including rtM204V/I, rtS202C/G/I, rtL180M, rtA181T/V, rtT184A/I/L/G/C/M, rtA194T, rtI169T, rtV173L, rtL80I, rtN236T, and PMID: 24790911
2014
Annals of laboratory medicine
Discussion: In our study, as expected, the rtM204I/V and rtL180M mutations related to lamivudine resistance were the most frequent, followed by the rtA181V/T mutation associated with adefovir resistance.
Discussion: The cases with the rtM204I
Discussion: reported that rtM204I mutation alone had lower replication competency than rtL180M/rtM204V, but clinically there was no difference in ALT, HBV DNA levels, and HBeAg positivity, similar to our data.
Molecular epidemiology and genotyping of hepatitis B virus of HBsAg-positive patients in Oman.
Abstract: Especially the lamivudine resistance mutations rtL180M/rtM204V and rtM204I were detected.
Discussion: Besides other previously described resistance mutations, the best-described drug resistance mutations are the LAM resistance mutations rtL180M and rtM204V/I and the adefovir resistance mutations rtA181V/T and rtN236T.
Discussion: The LAM mutations rtM204V/I are mapped to the catalytic center of
Viral evolutionary changes during tenofovir treatment in a chronic hepatitis B patient with sequential nucleos(t)ide therapy.
Abstract: A 54-year-old man diagnosed with HBeAg-positive chronic hepatitis B (CHB) was treated with entecavir (ETV) 1mg/day following an initial unsuccessful lamivudine (LAM) treatment (rtL180M, rtM204V/I).
Abstract: However, this patient experienced virological breakthrough under TDF with a HBV strain bearing rtL80M, rtL180M, rtM204V/I, rtA200V, rtF221Y, rt
ViMRT
HBV mutation literature information.
PMID: 
2014
Gastroenterology
