Sequential analysis of amino acid substitutions with hepatitis B virus in association with nucleoside/nucleotide analog treatment detected by deep sequencing.
Abstract: The lamivudine-resistant mutations at rtL180M and rtM204V and the entecavir-resistant mutation at rtT184L were detected in the first subject.
Randomized comparison of tenofovir disoproxil fumarate vs emtricitabine and tenofovir disoproxil fumarate in patients with lamivudine-resistant chronic hepatitis B.
Abstract: Patients were hepatitis B e antigen (HBeAg)-positive or HBeAg-negative, with levels of HBV DNA >=3 log10 IU/mL and lamivudine resistance mutations (HBV polymerase or reverse transcriptase amino acid substitutions rtM204I/V +- rtL180M by INNO-LiPA Multi-DR v3; Innogenetics, Inc, Alpharetta, GA).
Drug-resistance associated mutations in polymerase (p) gene of hepatitis B virus isolated from malaysian HBV carriers.
Abstract: A number of significant drug resistant mutations were found in five patients including S202I, N236T, M250L, L180M/V, M204I, A181T, T184G, M250V, and V173L.
Abstract: Of these, L180M/V and M204I were most frequently detected (80%) and associated with lamivudine in combination with emtricitabine and telbivudine drug resistance.
Discussion: Adefovir resistance was caused by N236T and /or A181V amino acid substitution, whereas entecavir resistance resulted from HBV reverse transcri
Generation of a human hepatoma cell line supporting efficient replication of a lamivudine resistant hepatitis B virus.
PMID: 24583110
2014
Journal of virological methods
Abstract: Accordingly, a human hepatoma (HepG2)-derived cell line was established by stable transfection of a plasmid containing a 1.2 unit length of HBV genome harboring rtL180M and rtM204V mutations that confer LAM resistance.
rtM204Q may serve as a novel lamivudine-resistance-associated mutation of hepatitis B virus.
Result: Clonal sequencing (44 clones) of the sample at this point (Sc2) showed predominance of rtM204Q strain (52%) concomitant with rtL180M+A181V (16%), rtA181T (16%), rtV173L+L180M+A181V (9%), rtA181V (5%) and wild-type (2%) strains in the viral pool.
Result: The rtM204Q emerged either alone or in concomitance with other mutations (rtM204I/V, rt
Establishment of real time allele specific locked nucleic acid quantitative PCR for detection of HBV YIDD (ATT) mutation and evaluation of its application.
Discussion: successfully developed an allele-specific quantitative PCR with combination of locked nucleic acid primers and a minor groove binder probe for the quantitative determination of minor viral quasispecies of the triple combination mutation rtV173L+rtL180M+rtM204V within one HBV genome.
Analysis of complete nucleotide sequences of Angolan hepatitis B virus isolates reveals the existence of a separate lineage within genotype E.
Result: HBV isolate LDA265 showed the lamivudine resistance substitution rtL180M associated to the adefovir resistance mutation rtA181V (not shown).
Result: The two others, LDA173 and LDA339, displayed the lamivudine resistance triple mutation rtV173L, rtL180M, rtM204V/I which causes the concomitant amino acid substitutions E164D/G and I195M in the small S protein (Table 3).
Stereoselective synthesis of 2'-fluoro-6'-methylene carbocyclic adenosine via Vince lactam.
PMID: 24697270
2014
The Journal of organic chemistry
Abstract: FMCA demonstrated excellent anti-HBV activity against both adefovir-resistant and lamivudine-resistant double (rtL180M/rtM204V) mutants as well as in lamivudine/entecavir triple mutants (L180M+S202G+M204V) in vitro.
Novel natural mutations in the hepatitis B virus reverse transcriptase domain associated with hepatocellular carcinoma.
Result: It is worth noting that we did not observe any of the common NA-related resistance mutations including rtM204V/I, rtS202C/G/I, rtL180M, rtA181T/V, rtT184A/I/L/G/C/M, rtA194T, rtI169T, rtV173L, rtL80I, rtN236T, and PMID: 24790911
2014
Annals of laboratory medicine
Abstract: The remaining showed the following mutation patterns: rtM204I/V (50.2%), rtL180M (39.2%), and rtA181T/V (19.6%).
Discussion: In our study, rtM204V was always accompanied by rtL180M.
Discussion: In our study, as expected, the rtM204I/V and rtL180M mutations related to lamivudine resistance were the most frequent, followed by the rtA181V/T mutation associated with adefovir resistance.
HBV mutation literature information.
PMID: 
2014
Hepatology research
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