In silico functional and structural characterization of hepatitis B virus PreS/S-gene in Iranian patients infected with chronic hepatitis B virus genotype D.
Introduction: The nucleotide substitutions in the pre-S1 promoter region, particularly the G2765A mutation, reduce the L protein expression and regulate the biological situation of HBV and result in a low viral load and then less serious disease in chronic HBV infection.
A substitution in the pre-S1 promoter region is associated with the viral regulation of hepatitis B virus.
Result: To further investigate the effect of the G2765A substitution on HBV production, we constructed a 1.3-fold HBV genome vector that enabled the production of HBV particles when transfected into HepG2 cells.
Discussion: Our results suggest the possibility that the Discussion: Reduced levels of L protein caused by the G2765A substitution may enable HBV to escape immune pressure, making it possible to persist in the hepatocytes of patients.
Discussion: Therefore, the G2765A substitution is considered to serve as a marker of good prognosis.
Discussion: proposed that either a sufficiently high or low L/S protein ratio inhibited virion secretion, and this was corroborated by our finding that patients infected with the G2765A mutant HBV showed a lower HBV DNA load.
HBV mutation literature information.
PMID: 
2020
Heliyon
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