Abstract: HBV monomers bearing BCP mutations A1762T/G1764A and A1762T/G1764A/C1766T, and precore mutations G1896A, G1899A and G1896A/G1899A, were transfected into HepG2 cells using a plasmid-free approach.
Lower mutation frequency of BCP/precore regions in e antigen-negative chronic HBV-infected children instead of adults patients.
Result: Notably, the frequency of the G1896A mutation in the HBeAg-negative child patients was significantly lower than in the HBeAg-negative
Table: G1899A
Discussion: Although the G1899A mutation only changes the glycine at codon 29 into aspartic acid and is always accompanied by the G1896A mutation, a previous study revealed that approximately 50% of the HBeAg negative variants contained this combined mutation.
Discussion: Similar features were also observed for other sites located in the BCP/precore regions(G1899A and A1846T, P<0.05)(Table 2).
Association of Mutations in the Basal Core Promoter and Pre-core Regions of the Hepatitis B Viral Genome and Longitudinal Changes in HBV Level in HBeAg Negative Individuals: Results From a Cohort Study in Northern Iran.
Abstract: We previously demonstrated that the accumulation of >= 6 mutations at eight key nucleotides located in these regions (G1613A, C1653T, T1753V, A1762T, G1764A, A1846T, G1896A, and G1899A) is a useful marker to predict the development of HCC regardless of advanced liver disease.
Phosphatase and tensin homologue genetic polymorphisms and their interactions with viral mutations on the risk of hepatocellular carcinoma.
Introduction: We and others have reported that HBV mutations C1653T, T1753V, A1762T/G1764A, T1674C/G, and C1766T/T1768A in the enhancer II/basal core promoter (EnhII/BCP) region; G1899A, C2002T, A2159G, A2189C, and G2203A/T in the precore/core region; as well as T53C, PMID: 26568165
2015
Scientific reports
Method: A risk prediction model to classify the HCC cases and controls was constructed according to the following steps: (1) Prediction factor selection: HBV genotype, the 11 independent HCC-related mutations (C1653T, C1673T, T1674C/G, C1730G, A1752G, T1753C, A1762T, G1764A, G1899A, G1915A/C and C1969T), the HLA SNPs (rs9272105 and rs9275319), the HCC-related multiplicative interactions (rs9272105 with the HBV genotype,
Precore/basal core promoter mutants quantification throughout phases of hepatitis B virus infection by Simpleprobe.
PMID: 26074702
2015
World journal of gastroenterology
Abstract: In five HBeAg-negative cases, we detected double mutation G1896A/G1899A.
Virological Characteristics of Acute Hepatitis B in Eastern India: Critical Differences with Chronic Infection.
Result: Major substitutions that were detectable in the BCP/PC region of acute isolates included T1753C (2.7%), A1762T/G1764A (8.1%), A1814C (2.7%), G1896A (10.8%) and G1899A (5.4%).
Result: Notably, the frequency of pre-core substitutions (A1814C, G1896A, and G1899A) was relatively high in acute infection reaching to almost half of that present in chronic infection.
Associations between hepatitis B virus basal core promoter/pre-core region mutations and the risk of acute-on-chronic liver failure: a meta-analysis.
Abstract: CONCLUSIONS: The HBV basal core promoter/pre-core mutations T1753V, A1762T, G1764A, C1766T, T1768A, A1846T, G1896A and G1899A, and an HBeAg-negative status correlate with an increased risk of HBV-ACLF.
Abstract: Several mutations were significantly correlated with ACLF: T1753V (1.889, 95 % confidence interval (CI) [1.357-2.631]), A1762T (2.696 [2.265-3.207]),