Method: Mutants sE2G, sL21R, sG24K, sT47A, sT47K, sC69* sL95W, sL98V, and sG145R were cloned into plasmid pBB4.5 1.2/PC, which contained a 1.2-fold length HBV genome of genotype C with a G1896A mutation in the preC region to facilitate the DNA replication in the in vitro system.
Hepatitis B virus infection in children of HBV-related chronic liver disease patients: a study of intra-familial HBV transmission.
Abstract: Recognized mutations associated with HBsAg detection and/or vaccination failure, T140I, T143S/M, G145R, and Y161F, were identified in 20 subjects; while mutations linked to HBeAg-defective variants, PC G1896A and BCP A1762T/G1764A, were found in 7 and 11 subjects, respectively.
Intergenotype recombinant analysis of full-length hepatitis B virus genomes from 516 Chinese patients with different illness categories.
Abstract: Difference in basal core promoter A1762T/G1764A mutations and precore G1896A mutation incidences was not significant between B/C recombinant and genotypes B or C virus, although the significance was there between genotypes B and C viruses.
Hepatitis B virus basal core promoter/precore mutants and association with liver cirrhosis in children with chronic hepatitis B virus infection.
PMID: 26577140
2016
Clinical microbiology and infection
Abstract: Among all the patients with genotype C viruses, the patients with LC had higher prevalence of C1653T, A1762T/G1764A and G1896A mutation frequency, higher hepatitis B e antigen (HBeAg) -negative rates, lower viral load, lower elevated alanine aminotransferase and lower anti-HBe positive rates than CHB patients.
Abstract: Patients with HBV genotype C viruses, high viral load and C1653T, A1762T/G1764A, G1896A mutant viruses, were more susceptible to developing PMID: 27340355
2016
World journal of gastroenterology
Abstract: Out of 240 CHB patients, 25 (10%) had C1653T and 33 (14%) had T1753V mutation in X region; 157 (65%) had A1762T/G1764A mutations in BCP region, 50 (21%) had G1896A mutation in precore region and 67 (28%) had pre-S deletions.
Precore/core region mutations of hepatitis B virus related to clinical severity.
PMID: 27158197
2016
World journal of gastroenterology
Abstract: Certain mutations, including preC G1896A, are also significantly related to HBeAg-negative chronic infection.
Hepatitis B virus basal core promoter mutations show lower replication fitness associated with cccDNA acetylation status.
Abstract: HBV monomers bearing BCP mutations A1762T/G1764A and A1762T/G1764A/C1766T, and precore mutations G1896A, G1899A and G1896A/G1899A, were transfected into HepG2 cells using a plasmid-free approach.
Association between hepatitis B virus basal core promoter/precore region mutations and the risk of hepatitis B-related acute-on-chronic liver failure in the Chinese population: an updated meta-analysis.
Abstract: CONCLUSIONS: HBV T1753V, A1762T/G1764A, A1846T, G1896A, and G1899A mutations are correlated with an increase in the risk of HB-ACLF.
Abstract: In sensitivity, specificity, and accuracy analysis, A1762T/G1764A had the highest sensitivity (67.43 %); A1762T/G1764A + G1896A triple mutations had the highest specificity (93.70 %); and T1753V + A1762T + G1764A mutation had the highest accuracy (65.42 %).
Different precore/core mutations of hepatitis B interact with, limit, or favor liver fibrosis severity.
PMID: 26992056
2016
Journal of gastroenterology and hepatology
Abstract: METHODS: Direct sequencing of the precore/core gene was used to detect A1762T/G1764A and G1757A mutations in the BCP and G1896A and G1899A mutations in the PC region.
Abstract: RESULTS: The prevalences of A1762T/G1764A, G1757A, G1896A, and G1899A mutations were 34.1%, 38.7%, 54.9%, and 29.3% (P < 0.001), respectively.
LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B.
PMID: 25968238
2016
Journal of clinical laboratory analysis
Abstract: BACKGROUND: Hepatitis B virus (HBV) G1896A mutation was associated with HBeAg seronegativity and hepatitis B related acute-on-chronic liver failure.
Abstract: CONCLUSION: The proposed Taqman-ARMS real-time PCR method for the detection of G1896A mutation of HBV was rapid, simple, sensitive, specific, and applicable in the clinical setting.
Abstract: In this study, we developed Taqman amplification refractory mutation system (Taqman-ARMS) and established a strict control system to detect HBV G1896A mutant.
Abstract: RESULTS: The assay has the sensitivity of 1E+3 IU/ml G1896A template, and 0.1% weak population virus with G1896A could be found in mixtures.
HBV mutation literature information.
PMID: 
2017
Journal of hepatology
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