Amino acid substitutions at positions 122 and 145 of hepatitis B virus surface antigen (HBsAg) determine the antigenicity and immunogenicity of HBsAg and influence in vivo HBsAg clearance.
Abstract: A variety of amino acid substitutions, such as K122I and G145R, have been identified around or within the a determinant of hepatitis B surface antigen (HBsAg), impair HBsAg secretion and antibody binding, and may be responsible for immune escape in patients.
Abstract: Genetic immunization in mice demonstrated that the priming of anti-HBs antibody response was strongly impaired by the substitutions K122I, G145R, and others, like G145I, G145W, and G145E.
Hepatitis B surface antigen variants in voluntary blood donors in Nanjing, China.
Abstract: Besides well known G145R, widely dispersed variations in the MHR of S region, were observed in 20 samples of all the strains sequenced.
Introduction: G145R was the major variation in the HBV isolates responsible for the occult HBV infections in Xiamen, China.
Result: G145R substitution, however, was not observed.
Result: Besides G145R, there were different types of aa substitutions in the MHR that were associated with lower reactivity in HBsAg assays reported in previous studies: i.e.
Result: The well-known G145R substitution was found in the 5 sequences, i.e., 531355, 546227, 563060, 506519, 525706, all of which were from B2/adw2.
Discussion: Besides of
High prevalence of occult hepatitis B virus infection in children born to HBsAg-positive mothers despite prophylaxis with hepatitis B vaccination and HBIG.
Abstract: Four out of the 13 mutations (C124R, C124Y, K141E, and D144A) strongly decreased the analytical sensitivity of seven commercial HBsAg immunoassays, and 10 (G119R, C124Y, I126S, Q129R, S136P, C139R, T140I, K141E, D144A, and G145R) significantly impaired virion and/or S protein secretion in both HuH7 cells and mice.
Ultra-deep pyrosequencing detects conserved genomic sites and quantifies linkage of drug-resistant amino acid changes in the hepatitis B virus genome.
4Method: In addition, to assess the changes in the most important epitopes of the overlapped S ORF, the fragment included the largest part possible of the C-terminal region of the immunodominant epitope ""a determinant"", which had the main immunotherapy escape mutation sG145R, associated with the rtW153Q NA compensatory variant."
5Result: In the ""a determinant"", only substitution s
8Discussion: Moreover, the only variant found in proportions above 0.1% was the well-known immune escape substitution sG145R, which modifies the antigenicity of the ""a determinant"", while viral particles remain infective."
A multicentre molecular analysis of hepatitis B and blood-borne virus coinfections in Viet Nam.
Abstract: In the immunodominant 'a' region of the surface gene, point mutations were identified in 31% (n = 58/187) of sequences, and 2.2% (n = 4/187) and 5
Result: Mutations associated with immune escape were identified in 23.5% (n = 45/187) samples and included the following residues Y100C (n = 6), T118K (n = 1), P120L/Q/S/T (n = 10), T123A/N (n = 2), I126N/S (n = 8), P127S (n = 2), Q129R (n = 2), G130D/N/R (n = 1), T131I (n = 4), M133L/T (n = 18), G144E (n = 1) and G145A/R (n = 4).
Hepatitis B virus gene mutations in liver diseases: a report from New Delhi.
Discussion: Due to the overlapping structure of HBV genomes, the most of RT-HBsAg concomitant mutations were found centered in the A-B interdomain, corresponding to the overlapping 'a' determinant with a cluster of affirmed immune-escape mutations, such as s126A/N/S, sT131N and sG145R.
Mutations associated with occult hepatitis B virus infection result in decreased surface antigen expression in vitro.
Discussion: The G145R mutation has been described as an immune escape mutant, as well as a potential contributor to lamivudine resistance; therefore, G145A may also represent a potential vaccine escape mutant.
Discussion: The G145R mutation has consistently resulted in decreased HBsAg levels and increased replication in lamivudine resistant mutants, especially among those with additional polymerase mutations, such as L180M and M204V.
Genotyping and molecular characterization of hepatitis B virus from human immunodeficiency virus-infected individuals in southern Africa.
Discussion: sQ164A, has been shown to reduce the antigenicity of HDV particles and sE164D, with the concomitant rtV173L, a lamivudine escape mutant, has reduced affinity for anti-HBs antibodies in vitro, similar to that of the classical G145R.
HBV mutation literature information.
PMID: 
2012
Journal of virology
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