Abstract: Based on this fact, we further investigated whether the mtHBsAg with the aa substitution G145R is able to induce mutant-specific antibody responses.
Abstract: One MAb recognized mtHBsAg with G145R but not wild type and other mtHBsAg.
Abstract: Strikingly, serum samples from mice immunized with mtHBsAg with G145R recognized plasma-derived mtHBsAg.
Abstract: The amino acid (aa) substitutions like G145R, F134S, and C147W affected the binding of anti-HBs antibodies to corresponding mtHBsAg to different extents.
Abstract: The impact of aa substitutions G145R and F134S on the immunogenicity was accessed by genetic immunization of mice with vectors
Molecular analysis of antigenicity and immunogenicity of a vaccine-induced escape mutant of hepatitis B virus.
Abstract: METHODS: Mutation from glycine to arginine at aa145 was introduced into replication-competent DNA of HBV.
Characterization of hepatitis B virus surface antigen and polymerase mutations in liver transplant recipients pre- and post-transplant.
PMID: 12780567
2003
American journal of transplantation
Abstract: All three patients with hepatitis B immune globulin (HBIG) treatment failure followed by nucleoside analogue treatment failure were infected with HBV genotype C; a pre-existing HBV S antigen (HBsAg) mutation (sD144A) was identified in one patient pretransplant, while sG145R mutations emerged in the other two patients post-transplant.
Molecular epidemiology and transmission of hepatitis B virus in close family contacts of HBV-related chronic liver disease patients.
Abstract: The specificity of intracellular HBsAg- anti-HBs interaction was further investigated in cells transfected with HBV genomes expressing wild-type HBsAg or immune escape HBsAg (with a G145R mutation).
Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.
Abstract: Furthermore, cotransfection studies were performed with the G145R variant and a Wt virus S-protein expressing construct and vice versa.
Abstract: Hepatitis B virus with a G145R mutation in the small surface protein is considered the quintessential immune escape mutant because it frequently is found in vaccinated individuals with breakthrough infections and liver transplant recipients under anti-hepatitis B surface antigen (HBsAg) immunoglobulin prophylaxis.
Abstract: However, because spread of a virus depends not only on immune pressure but also on the viral phenotype, we investigated the biologic properties of the G145R variant.
Abstract: In conclusion, the significant defect of the G145R<
Mutations of the surface protein of hepatitis B virus.
Abstract: The G145R mutant is replication competent and is stable, and it appears to be the most common variant.
Reduced antigenicity of the hepatitis B virus HBsAg protein arising as a consequence of sequence changes in the overlapping polymerase gene that are selected by lamivudine therapy.
Abstract: HBsAg proteins containing the vaccine escape mutations G145R and D144E/G145R demonstrated markedly reduced binding to anti-HBs antibody.
Occult HBV infection in cryptogenic liver cirrhosis in an area with high prevalence of HBV infection.
PMID: 12014730
2002
The American journal of gastroenterology
Abstract: a determinant mutations were detected in two patients in group I (K122N and G145R, C125A) and one patient in group II (1126N).
Sensitive detection of HBsAg mutants by a gap ligase chain reaction assay.
PMID: 12089278
2002
Journal of clinical microbiology
Abstract: Detection of the G145R mutant in clinical specimens was evaluated by testing 56 suspect serum specimens.
Abstract: The G145R mutation was most frequently observed in specimens producing a diagnostic anomaly or from transplant patients but was also observed in specimens from vaccinated individuals and specimens in which HBsAg diagnostic escape was suspected.
Abstract: The G145R mutation was observed in 18 of 28 HBV-DNA-positive samples.
Abstract: The most common mutation observed in these variants is a glycine-to-arginine substitution at amino acid 145 (G145R).
Abstract: Therefore, the gLCR assay is a sensitive and specific method for detection of G145R mutants, which could be modified to include the detection of
HBV mutation literature information.
PMID: 
2004
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