Result: The alternate orientation of F97 in L60V-HBc increases the adjacent hydrophobic pocket similar to the smaller side-chain in F97L-HBc does, which also points away from the dimer axis (Figure 3g).
Result: While L60V-HBc, WT-HBc and F97L-HBc had similar transition temperatures between 91 C and 95 C, the transition temperature of P5T-HBc was much lower (TM = 86 C), showing that the mutation P5T weakens the stability of the whole capsid substantially.
Discussion: Compared to the WT-HBc and
Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97.
Introduction: Naturally occurring point mutations like HBc-F/I97L with an immature secretion phenotype or point mutations with a low
Introduction: We asked what the underlying conformational changes were and whether these changes differed between CLPs of wt HBc, mutants with immature secretion phenotype (HBc-F97L) or mutants with low secretion phenotypes (HBc-P5Tand HBc-L60V).
Result: In contrast, the map of HBc-F97L (EMDB 4417 and all maps generated in this study (Table 1), (Figure 6) were reconstructed from HBc-CLP purifications without TX100 (and any other detergents) and did not show a pocket factor.
A nuanced role of the small loop of hepatitis B virus small envelope protein in virion morphogenesis and secretion.
8Discussion: Naturally occurring core mutants I97L (isoleucine to leucine) or F97L (phenylalanine to leucine), exhibited a so-called ""immature secretion"" phenotype, which allows excessive secretion of virions containing an immature genome."
Naturally occurring core protein mutations compensate for the reduced replication fitness of a lamivudine-resistant HBV isolate.
Introduction: Previous studies have shown that the substitution of proline (P) with threonine (T) at codon 5 of HBcAg caused lower levels of virion secretion, which, would revert to the wild type secretion phenotype when it coexisted with F97L mutation.
Persistence of Hepatitis B Virus DNA and the Tempos between Virion Secretion and Genome Maturation in a Mouse Model.
Result: It is reminiscent of HBc mutant F97L, which exhibited a pleiotropic phenotype, including both a cis-defect in viral DNA synthesis and a trans-defect in virion secretion (; see also the Discussion).
Discussion: Indeed, in our previous study, when virion secretion of mutant F97L was genetically blocked with another surface antigen knockout mutation (SK/O), intracellular genome maturation to full-length RC DNA was well restored (~30%) in the double mutant F97L/SK/O.
Discussion: It remains to be investigated whether these in vitro capsid assembly properties of mutant F97L could also influence the efficiency of envelopment in virion secretion in mouse models.
Discussion: It was reported that HBc
Novel point and combo-mutations in the genome of hepatitis B virus-genotype D: characterization and impact on liver disease progression to hepatocellular carcinoma.
8Discussion: Interestingly, the most frequently observed naturally occurring core protein mutation found in CHB and also in HCC patients I97L or F97L which is associated with ""immature virion secretion"" was completely absent in Indian patients with HBV-genotype D while among the ""low secretion core variants"" such as P5T/A/S, a new variant P5R was noted with high frequency in HBV-genotype D related HCC in previous Indian study."
Chimeric constructs between two hepatitis B virus genomes confirm transcriptional impact of core promoter mutations and reveal multiple effects of core gene mutations.
Abstract: Since the structures of the mutant and wild-type capsids are essentially the same and the mutation is not involved in the contact between dimers, we suggest that the F97L mutation affects the dynamic behavior of dimer and capsid.
Abstract: We examined in vitro assembly of empty HBV capsids from wild-type and F97L core protein assembly domains.
Influence of a putative intermolecular interaction between core and the pre-S1 domain of the large envelope protein on hepatitis B virus secretion.
Abstract: In addition, the secretion kinetics of the mature genomes are comparable between the wild-type HBV and the mutant F97L.
Abstract: In this study, our kinetic analysis of virion secretion of the mutant F97L (phenylalanine to leucine) indicates that the secretion of its immature genome does not occur earlier than that of its mature genome.
Abstract: Therefore, the immature secretion phenomenon of mutant F97L is not caused by premature secretion or more efficient secretion.
The mechanism of an immature secretion phenotype of a highly frequent naturally occurring missense mutation at codon 97 of human hepatitis B virus core antigen.
HBV mutation literature information.
PMID: 
2021
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