Abstract: Primary drug resistance mutations such as rtI169T, rtA181T/V, rtT184A/C/F/G/I/L/M/S, rtA194T, rtS202C/G/I, rtM204I/V/S, rtN236T, rt M250I/L/V and rtV173L were not detected in any of the patient samples.
Mutations in reverse transcriptase region of HBV affect Hepatitis B surface antigen titers and its correlation with HBV DNA.
PMID: 32087080
2019
Journal of infection in developing countries
Abstract: HBsAg was positively correlated with HBV DNA levels in the wild-type group (r = 0.322, p < 0.01), as well as in the M204I/V, L180M+M204I/V, A181T/V, and N236T subgroups, while no correlation was found in the A181T/V+N236T subgroup (r = 0.159, p = 0.217).
Abstract: For patients with A181T/V or N236T mutation, HBsAg was positively correlated with HBV DNA in older patients (>= 40 years), but not in younger patients (< 40 years).
Abstract: RESULTS: HBsAg was lower in the wild-type and A181T/V
Potential resistant mutations within HBV reverse transcriptase sequences in nucleos(t)ide analogues-experienced patients with hepatitis B virus infection.
Introduction: These two categories are known as classical mutations, which include: (i) M204I/V mutation, which associates with LAM or LDT resistance; (ii) N236T or A181T/V mutations, which associate with ADV resistance; (iii) M204V + L180M and either T184A/G/I/L/S or S202G or M250V to develop resistance to ETV.
Result: Interestingly, mutation pattern of A181T associated to F221Y was detected after 22 months of TDF monotherapy with virological and biochemical breakthrough in case two.
Result: Out of 24 mutations of L229 (Table 3), 18 were associated to M204I or V
Characterization and Clinical Significance of Natural Variability in Hepatitis B Virus Reverse Transcriptase in Treatment-Naive Chinese Patients by Sanger Sequencing and Next-Generation Sequencing.
PMID: 31189581
2019
Journal of clinical microbiology
Discussion: Currently, the well-known classical NA resistance mutations are mainly located in domains B, C, D, and E, such as rtI169T, rtA181T/V, and rtT184A/C/F/G/I/L/M (located in domain B), rtS202C/G/I and rtM204I/V/S (located in domain C), rtN236T (located in domain D), and rtM250I/L/V (located in domain E).
Discussion: In this study, except rtA181T,
Complementation of Wild-Type and Drug-Resistant Hepatitis B Virus Genomes to Maintain Viral Replication and Rescue Virion Production under Nucleos(t)ide Analogs.
Abstract: In the present study, HBV genomes with frequently detected reverse transcriptase (RT)/surface truncation MTs, rtA181T/sW172*, rtV191I/sW182* and rtM204I/sW196*, were phenotypically characterized alone or together with their WT counterparts in different ratios by transient transfection in the absence or presence of NAs.
Hepatitis B infection among HIV infected individuals in Gabon: Occult hepatitis B enhances HBV DNA prevalence.
Method: As previously described, we used the Mutation Reporter Tool (MRT) software (http://hvdr.bioinf.wits.ac.za/mrt/) to look for HBV resistance-associated mutations (RAMs) in the Polymerase catalytic domain represented by major RAMs (A181T/V/S, A194T, M204V/I/S and N236T) and compensatory RAMs (I169T, V173L, L180M, S202G/I and M250V.
Hepatitis B virus rtA181T/sW172non-stop mutation may increase resistance fold to adefovir- and entecavir-resistant mutants compared to rtA181T/sW172* mutation.
Abstract: 3.69-fold in half maximal effective concentration of wild-type strain); rtA181T/sW172L + rtS202G + rtM204V strain exhibited higher HBV DNA production and entecavir resistance fold than that of rtA181T/sW172* + rtS202G + rtM204V strain (50.98% vs.
Abstract: 44.33% (524/1182) rtA181T-positive samples were detected with signature drug-resistant mutations,
The truncated mutant HBsAg expression increases the tumorigenesis of hepatitis B virus by regulating TGF-beta/Smad signaling pathway.
Method: Additionally, some of above BALB/c nude mice were also given TGFbeta1(Peprotech, UK) at the dose of 2 ng/ml 20 g via tail vein injection to clarify whether TGF-beta/Smad pathway was involved in the tumorigenicity of HBV-rtA181T/sW172* mutant.
Method: The first part was to investigate and compare the tumorigenesis of different overlapping S gene mutation (sW172L and sW172*) on rtA181T mutant strain in vitro and in vivo.
Discussion: And this finding is also consistent with the clinical increased risk of developing HCC in PMID: 29654894
2018
International journal of antimicrobial agents
Abstract: Moreover, A181T/V, ETV resistance mutations and multidrug resistance mutations were found more frequently in HBV genotype C compared with genotype B (21.2% vs.
Evolution of drug-resistant mutations in HBV genomes in patients with treatment failure during the past seven years (2010-2016).
Abstract: Among the primary NA-resistant mutations, rtM204I (13.44%, 545/4055) occurred more frequently, followed by rtM204V, rtN236T, rtA181T, and rtA181V.
Abstract: Frequencies of rtL180M and rtA181T/V increased gradually in the past seven years, to which we should pay more attention.
HBV mutation literature information.
PMID: 
2019
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