Abstract: The newly identified EBV subtypes, which contains four Chinese-specific NPC-associated amino acid substitutions (BALF2 V317M, BNRF1 G696R, V1222I and RPMS1 D51E), showed a robust positive association with the risk of NPC in China (Odds Ratio = 4.80, 20.00, 18.24 and 32.00 for 1, 2, 3 and 4 substitutions, respectively, P trend <0.001).

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Method: In the present study, a novel mutation (BNRF1 V1222I, loc5399) was validated via nested PCR and Sanger sequencing using mouthwashes from 108