Result: After titrating the concentrations of 8his-tagged sACE22(WT) and sACE22.v2.4 and measuring bound protein to S-expressing cells by flow cytometry, it was found S-N501W and S-N501Y do show enhanced specificity for wild type sACE22, but the effect is small and sACE22.v2.4 remains the stronger binder (Figure 5C).
Result: BLI kinetics between immobilized sACE22-IgG1 and monomeric RBD as the analyte showed reduced affinity of a representative mutant, RBD-Y449K, to both wild type and engineered sACE22 (Table 1).
Result: Both N501W and N501Y mutants of SARS-CoV-2 RBD displayed increased affinity fo
SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma.
Abstract: At day 73, an E484K substitution in the receptor-binding domain (RBD) occurred, followed at day 80 by an insertion in the NTD N5 loop containing a new glycan sequon, which generated a variant completely resistant to plasma neutralization.
Introduction: Four plasma samples did not show neutralization activity against the SARS-CoV-2 WT and SARS-CoV-2 D614G variant.
Introduction: In contrast, the single mutation in the RBD (E484K) swaps the charge of the sidechain, which would significantly alter the electrostatic complementarity of antibody binding to this region.
Introduction: In the RBD, the possibility to escape is limited and the mutation E484K that we found is one of the most frequent mutations to escape monoclonal
Neutralising antibodies in Spike mediated SARS-CoV-2 adaptation.
Introduction: Another European cluster in GISAID includes DeltaH69/DeltaV70 along with the RBD mutation N439K.
Introduction: Here we document real time SARS-CoV-2 emergence of DeltaH69/DeltaV70 in combination with the S2 mutation D796H following convalescent plasma therapy in an immunocompromised human host, demonstrating selection and reduced phenotypic susceptiblility of selected mutations.
Introduction: The capacity for successful adaptation is exemplified by the Spike D614G mutation, that arose in China and rapidly spread worldwide, now accounting for more than 90% of infections.
Introduction: There it was associated with the RBD mutation Y453F in almost 200 individuals.
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Abstract: Among the numerous mutations detected, 14,408C>T and 23,403A>G mutations resulting in RNA-dependent RNA polyme
Introduction: However, some studies suggested that D614G mutation in the spike protein may contribute to increased infectivity or transmissibility of SARS-CoV-2 leading to increased severity of COVID-19.
Introduction: The D614G mutation of the spike protein was observed sometimes in late January 2020 both in Europe and in China, but then this mutation spread first in the Europe and gradually globally.
Introduction: Thus the distribution of spike protein D614G mutation has temporal and geographical variation.
The Novel Coronavirus Enigma: Phylogeny and Analyses of Coevolving Mutations Among the SARS-CoV-2 Viruses Circulating in India.
PMID: 33496683
2020
JMIR bioinformatics and biotechnology
Abstract: All the minor group mutations, except 11083G>T (L37F, NSP6 gene), were unique to the Indian isolates.
Abstract: The secondary structure of the RNA-dependent RNA polymerase/nonstructural protein NSP12 was predicted with respect to the novel A97V mutation.
Result: A mutation in the S gene, G1167V (Glycine to Valine) was identified in Oklahoma-ADDL-4.
Result: A previously reported deleterious variation in the protein expressed from ORF3a, Q57H, was recorded in Oklahoma-ADDL-1, the genome isolated at the beginning of the pandemic in Oklahoma.
Result: A previously reported mutation in S gene - D614G (Aspartate to Glycine), was identified in all the genomes sequenced.
Result: A previously reported mutation in the ORF1ab gene P4715L) (Proline to Leucine) was recorded alongside novel mutations at various amino acid locations.
SARS_CoV_2 mutation literature information.
PMID: 
2020
bioRxiv
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