Result: All animals had high IgG antibody titers against the S-614G antigen as measured by an ELISA (7-month GMT = 32,510; 22-month GMT = 34,443) with 1.4- and 3.4-fold decreases in IgG antibody titers against the Omicron spike antigen (7-month GMT = 22,988; 22-month GMT = 10,159) (Figure 2E).
Result: All animals had measurable IgG antibody titers against the spike (S-614G) with approximately 2.6-fold higher antibody titers after two vaccinations (geometric mean titer [GMT] = 8,611, compared to one vaccination: GMT = 3,620).
Result: Animals were first infected with a S-614G isolate (n = 6; 7 months prior) or an original Wuhan-like S-614D i
Mutation rate of SARS-CoV-2 and emergence of mutators during experimental evolution.
PMID: 35419205
2022
Evolution, medicine, and public health
Abstract: Methodology: We performed experimental evolution with two strains of SARS-CoV-2, one carrying the originally described spike protein (CoV-2-D) and another carrying the D614G mutation that has spread worldwide (CoV-2-G).
Conclusion: Assuming that the D614G genotype is better adapted than its ancestor in Vero cells, this observation is consistent with the hypothesis that fitter genotypes adapt at a slower pace.
Conclusion: Here, we followed the evolution in cells of two strains of SARS-CoV-2: one with the original spike protein (CoV-2-D) and one carrying the D614G mutation (CoV-2-G), a variant that emerged in the early phase of the pandemic and soon became prevalent.
Conclusion: The two genomic backgrounds shared several de novo mutations, suggesting that the D614G
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Abstract: Many laboratories are using previously developed L452R-specific PCRs to discriminate Omicron from Delta mutations, but these tests may be unreliable.
Abstract: We report 25 cases of infection with SARS-CoV-2 Omicron variant containing spike protein L452R mutation in northern Lombardy, Italy.
Development of antibody resistance in emerging mutant strains of SARS CoV-2: Impediment for COVID-19 vaccines.
Abstract: Lower than expected inhibitory activity of antibodies was reported against viruses with E484K Spike mutation, including B.1.1.7 (UK), P.1 (Brazil), B.1.351 (South African), and new Omicron variant (B.1.1.529) with E484A mutation.
Molecular Dynamics and MM-PBSA Analysis of the SARS-CoV-2 Gamma Variant in Complex with the hACE-2 Receptor.
Introduction: Additionally, the mutation N501Y exhibits a compensatory effect since it favors pi-pi interaction with the residue Y41.
Introduction: The alpha variant has three mutations of interest in the spike protein: (i) the N501Y mutation that corresponds to the receptor-binding motif (RBM); (ii) a 69/70 deletion in the receptor binding domain (RBD) that triggers a notable change in the conformation of the RBM; and (iii) the P681H mutation found near the furin S1/S2 cleavage site.
Introduction: The beta variant, also known as 20H/501Y.V2, was identified in South Africa and is characterized by carrying the K417N, E484K and N501Y mutations in the RBM of the
Potent Anti-SARS-CoV-2 Efficacy of COVID-19 Hyperimmune Globulin from Vaccine-Immunized Plasma.
Abstract: Pseudotyped and authentic virus-based assays show that COVID-HIG displays broad-spectrum neutralization effects on a wide variety of SARS-CoV-2 variants, including D614G, Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Kappa (B.1.617.1), Delta (B.1.617.2), and Omicron (B.1.1.529) in vitro.
COVID-19 outbreak in Malaysia: Decoding D614G mutation of SARS-CoV-2 virus isolated from an asymptomatic case in Pahang.
Abstract: FINDINGS: The disease characteristics of the Omicron (R346K) variant were found to be similar to that of the Delta variant infection in hamsters like viral replication in the respiratory tract and interstitial pneumonia.
Abstract: INTERPRETATION: This preliminary data shows that Omicron (R346K) variant infection can produce moderate to severe lung disease similar to that of the Delta variant and the neutralizing antibodies produced in response to Omicron (R346K) variant infection shows poor neutralizing ability against other co-circulating SARS-CoV-2 variants like Delta which necessitates caution as it may lead to increased cases of reinfection.
Abstract: Neutralizing antibody response against Omicron (R346K) variant was detected from day 5 and th
Split T7 promoter-based isothermal transcription amplification for one-step fluorescence detection of SARS-CoV-2 and emerging variants.
Introduction: D614G), conferring greater infectivity and more rapid spread, have become predominant in various regions.
Introduction: Additionally, variants of SARS-CoV-2 with the 23,403 A > G mutation (p.
Introduction: Moreover, STAR was utilized for multiplex detection of the D614G mutation and N gene of SARS-CoV-2 in a single tube as well as for the direct detection of bacterial 16S rRNA without additional nucleic acid purification, thereby confirming the wide applicability of this method for nucleic acid biomarker detection.
Result: 5B indicate that G at N4 minimized the background noise in the presence of the wild-type target while generating a high fluorescence signal in the presence of the mutant target (D614G).
Result: Furthermore, the sensitivities for both targets were evaluated, con
Increased Secondary Attack Rate among Unvaccinated Household Contacts of Coronavirus Disease 2019 Patients with Delta Variant in Japan.
PMID: 35409572
2022
International journal of environmental research and public health
Abstract: We studied household contacts of index cases of COVID-19 infected with Delta (L452R mutation), Alpha (N501Y mutation), and wild strain from December 2020 through November 2021 in Itako, Japan.
Introduction: SARS-CoV-2 VOCs bearing the L452R spike protein mutation demonstrate increased transmissibility, infectivity, and avoidance of antibody neutralization.
Method: Almost all cases with the N501Y mutation during the study period in Japan were confirmed to be the Alpha variant by RNA sequencing.
Method: Among unvaccinated Discussion: Last, the Delta and Alpha variants were mainly confirmed by the L452R and N501Y mutations, respectively.
SARS_CoV_2 mutation literature information.
PMID: 
2022
Microbiology spectrum
Browser Board
Co-occurred Entities
Filtrator
ViMRT