Result: Figure 2 represents snapshot images obtained from the MD simulations for the WT and H274Y mutant NA-OTV complexes, showing the OTV binding site and the region adjacent to residue 274.
Result: Figure 3 shows the dRIN graphs for the WT and H274Y mutant NA complexed with OTV.
Result: Figure 4B indicates that the occupancy of ligand-residue interactions between the OTV and its surrounding residues, E119, D151, R152, and S246 decreased after H274Y mutation, whereas the occupancy of the OTV-Y406 interaction increased.
Result: Figure 4B shows the change in the occupancies of residues interacting with each of the WT and H274Y mutant
Result: dRIN of H274Y mutant NA.
Identification of H3N2 NA and PB1-F2 genetic variants and their association with disease symptoms during the 2014-15 influenza season.
Abstract: In human nasal epithelial cell cultures, a virus with the novel NAg+F2P genotype replicated less well compared with a virus with the parental genotype.
Abstract: Retrospective analyses of clinical data showed that NAg+F2P genotype viruses were associated with increased cough and shortness of breath in infected patients.
Abstract: The NA and PB1-F2 mutations were present in a subset of clade 3C.2a viruses (NAg+F2P), which dominated during the subsequent influenza seasons.
Abstract: The isolates were classified by HA clade and the presence of a new set of co-selected mutations in NA (a glycosylation site, NAg+) and PB1-F2 (H75P).
Method: A/Colu
Molecular Characterization of Seasonal Influenza A and B from Hospitalized Patients in Thailand in 2018-2019.
Result: Additional amino acid substitutions were found at P99S, C123S, and S139C for segment NA, T285I for segment M, and V453A at the NP segment (Tables S7 and S8).
Result: Among these Thai strains, we found additional mutations at antigenic sites; S91R (Cb), S181T(Sa), and T202I (Sb) (Figure 2B).
Result: Determination of amino acid substitutions among the A/H1N1 isolates also revealed the mutation Q51K, F74S, G77R, V81A, I188T
Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y.
Abstract: The 2018 virus harbored amino acid mutations (I123V and N205S) in important functional sites; however, 108R and 189G were highly conserved between A/California/07/2009 and the 2018 variant.
Abstract: To better understand interactions between influenza viruses and the human innate immune system, we generated and rescued seasonal 2009 H1N1 IAV mutants expressing an NS1 protein harboring a dual mutation (R108K/G189D) at these conserved residues and then analyzed its biological characteristics.
Introduction: Here, we sequenced a seasonal 2009 H1N1 influenza virus isolated in 2018 and confirmed the presence of mutations I123V and N205S in important functional sites.
Introduction: However, the NS
Novel Clade 2.3.4.4b Highly Pathogenic Avian Influenza A H5N8 and H5N5 Viruses in Denmark, 2020.
Abstract: Genetic analyses of one of the H5N8 viruses revealed the presence of a substitution (PB2-M64T) that is highly conserved in human seasonal influenza A viruses.
Result: In addition, one of the H5N8 viruses (A/barnacle goose/Denmark/14139-3/2020) had a PB2-M64T amino acid substitution that is highly conserved in human influenza A H1N1, H2N2, and H3N2 viruses.
Result: The PB2-M64T substitution was not present in A/Astrakhan/3212/2020(H5N8) (EPI_ISL_1038924) virus detected in workers at a poultry farm in Russia on 12 December 2020.
Discussion: The genetic characterization revealed that one of the Danish H5N8 viruses contained a PB2-M64T substitution.
Bilobetin, a novel small molecule inhibitor targeting influenza virus polymerase acidic (PA) endonuclease was screened from plant extracts.
Abstract: Dose-dependent inhibition assay showed that Epimedii folium can effectivity inhibit the PAN and PAN-I38T with IC50 of 11.23 and 26.03 muM, respectively.
Abstract: In 45 kinds of plant extracts, eight can effectively inhibit the PAN and PAN-I38T mutant in the primary screening.
Abstract: folium were virtually screened using the in silico method, and the compounds ginkgetin and bilobetin bind to the active pocket of PAN and PAN-I38T with a strong interaction force.
Cell culture-based production and in vivo characterization of purely clonal defective interfering influenza virus particles.
Abstract: An NA-N293/294S substitution was not present in sequences from the GISAID database.
Abstract: Fewer than 1% of analyzed viruses had amino acid substitutions associated with reduced susceptibility to baloxavir (PA-E199G, PA-E199E/G) or reduced or highly reduced inhibition by neuraminidase inhibitors (NA-R150/152K, NA-I221/222M, NA-I221/222I/M, NA-I221/222V, NA-H275Y.
Abstract: Co-occurrence of the mutations D222G and D222N was detected in a substantial number of the studied fatal cases (41%).
Abstract: The D222G/N mutations in the hemagglutinin (HA) gene of A(H1N1)pdm09 are associated with severe and fatal human influenza cases.
Abstract: The D222G/N mutations were detected at a low frequency (less than 1%) in the rest of the studied samples from fatal and nonfatal cases of influenza.
Abstract: The high rate of occurrence of HA D222G/N mutations in A(H1N1)pdm09 viruses, their increased ability to replicate in the LRT and their association with fatal outcomes points to the importance of monitor
Investigating the sequence variation in the influenza A matrix genes during the 2017-2018 and 2018-2019 seasons in samples from a local population in London.
PMID: 34339766
2021
Journal of virological methods
Abstract: All samples displayed the core mutations for H1N1 M1(C154T; G174A and G238A) and for H3N2 M1(C153T; C163T and G189T); three of the H1N1pdm09 viruses also showed a small number of point mutations.
IV mutation literature information.
PMID: 
2021
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