Abstract: Of the three patients infected with wild-type influenza virus, two cleared the virus after baloxavir treatment, while the third patient developed the polymerase I38T variant linked to baloxavir resistance.
Abstract: RESULTS: Two patients were infected with influenza A/H1pdm09 carrying a neuraminidase variant (H275Y) linked to oseltamivir resistance.
Acetylation at K108 of the NS1 protein is important for the replication and virulence of influenza virus.
Abstract: In vitro and mouse studies showed that the deacetylation-mimic mutation K108R in the NS1 protein attenuated the replication and virulence of WSN-NS1-108R, while the constant acetylation-mimic mutant virus WSN-NS1-108Q showed similar replication and pathogenicity as the wild-type WSN virus (WSN-wt).
Abstract: To further explore the function of the K108 acetylation modification of the NS1 protein, a deacetylation-mimic mutation (K108R) and a constant acetylation-mimic mutation (K108Q) were introduced into the NS1 protein in the background of A/WSN/1933 H1N1 (WSN), resulting in two mutant viruses (WSN-NS1-108R and WSN-NS1-108Q).
Optimization of 4-Aminopiperidines as Inhibitors of Influenza A Viral Entry That Are Synergistic with Oseltamivir.
PMID: 32069052
2020
Journal of medicinal chemistry
Abstract: Compound 16 displayed a significant decrease of viral titer when evaluated in the infectious assays with influenza virus H1N1 (A/Puerto Rico/8/1934) or H5N1 (A/Vietnam/1203/2004) strains and the oseltamivir-resistant strain with the most common H274Y mutation.
Identification of a Permissive Secondary Mutation That Restores the Enzymatic Activity of Oseltamivir Resistance Mutation H275Y.
PMID: 32064779
2020
Influenza and other respiratory viruses
Abstract: In vitro studies have revealed that an I38T substitution in the polymerase acidic subunit (PA) is associated with reduced susceptibility of influenza viruses to baloxavir.
Abstract: METHODS: Three assays were developed based on RNase H2-dependent PCR (rhPCR) and named A/H1pdm PA_I38T rhPCR, A/H3 PA_I38T rhPCR, and B PA_I38T rhPCR.
Abstract: OBJECTIVES: Development of a rapid and simple method for monitoring influenza A(H1N1)pdm09, A(H3N2), and B viruses possessing the I38T substitution in PA.
Method: The resulting PCR product of A/H3 was cloned using a TOPO TA Cloning Kit for Sequenci
Filamentous versus Spherical Morphology: A Case Study of the Recombinant A/WSN/33 (H1N1) Virus.
Abstract: Bioinformatics algorithms predicted a neutral structural effect of the Ser126Cys mutation.
Abstract: Next-generation sequencing (NGS) suggested a rare Ser126Cys substitution in the M1 protein of rWSN, which was confirmed by the mass spectrometric analysis.
Abstract: The mrWSN_M1_126S virus generated after the introduction of the reverse Cys126Ser substitution exhibited a similar host-dependent partially filamentous phenotype.
Reassortment and adaptive mutations of an emerging avian influenza virus H7N4 subtype in China.
Result: A unique feature of JS2018 among the novel H7N4 isolates was its E627K in PB2, similar to the H7N9 virus, a mutation that is thought to increase virulence.
Result: Again, JS2018 was the only H7N4 isolate with the E627K mutation in PB2 which was also present in human H7N9 viruses.
Result: In addition, a PB2 K683T mutation occurred in JS2018 and Ck2018 but not in the duck H7N4 viruses.
Result: None of the novel H7N4 viruses would be resistant to amantadine as no S31N in M2 was observed.
Result: Of special note, four mutations in the JS2018 HA (H242Q, N283D, H275Y.
Abstract: Binding preference of mutant HAs revealed that an E190V substitution is critical for the recognition of sulfated SAalpha2,3Gal glycans.
Abstract: It was revealed that E190V and N192D substitutions of the HA increased the recovery of viruses derived from an H6 duck virus isolate, A/duck/Hong Kong/960/1980 (H6N2), in chickens.
H7N9 Influenza Virus Containing a Polybasic HA Cleavage Site Requires Minimal Host Adaptation to Obtain a Highly Pathogenic Disease Phenotype in Mice.
Abstract: In addition to the PBC site, we identified three other mutations that are important for host-adaptation and virulence in mice: HA (A452T), PA (D347G), and PB2 (M483K).
Method: Amino acid mutations in HA (A452T, H7 HA numbering with the signal peptide included), PA (D347G), and PB2 (M483K) were also inserted into each respective pPol vector by PCR using PrimeSTAR Max and in-fusion cloning.
Discussion: A previous study identified a D347N mutation in the
Multiple Border-Zone Infarcts Triggered by Influenza A Virus Infection in a Patient With Cerebral Autosomal Dominant Arteriopathy Presenting With Subcortical Infarcts and Leukoencephalopathy.
PMID: 32102741
2020
Journal of stroke and cerebrovascular diseases
Abstract: Gene analysis identified a heterozygous mutation c.160C>T in exon 2 of the NOTCH3 gene (p.Arg54Cys).
Antigenic variants of influenza B viruses isolated in Japan during the 2017-2018 and 2018-2019 influenza seasons.
PMID: 31955521
2020
Influenza and other respiratory viruses
Abstract: One B/Yamagata-lineage isolate carrying a G407S mutation in its NA showed a marked reduction in susceptibility to zanamivir, peramivir, and laninamivir.
Discussion: Further investigations are required to determine whether this NA-G407S mutation affects the transmissibility of influenza B viruses.
Discussion: Influenza B viruses carrying the G407S mutation in their NA, which confers reduced susceptibility to NA inhibitors, were sporadically detected in Japan during the 2004-2005 season15 and in Australia and the United States during the 2015-2016 season.23 In this study, we also detected an influenza B virus with the NA-G407S mutation during the 2017-2018 seas
IV mutation literature information.
PMID: 
2020
Transplant infectious disease
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