Abstract: Here, we demonstrate that antagonist activity of P6A is associated with low affinity of the A6 alphabetaTCR for Tax-P6A/HLA-A2.
Abstract: The structure of the A6 alphabetaTCR/HTLV-1 Tax-peptide/MHC I complex with proline 6 of Tax substituted with alanine (P6A), an antagonist, is nearly identical to the structure with wild-type Tax agonist.
Distinct p300-responsive mechanisms promote caspase-dependent apoptosis by human T-cell lymphotropic virus type 1 Tax protein.
PMID: 11046153
2000
Molecular and cellular biology
Abstract: Importantly, nuclear expression of the minimal CBP/p300-binding peptide of Tax induced apoptosis in the absence of Tax-dependent transcriptional activities, while its K88A counterpart did not cause cell death.
Abstract: The CBP/p300-binding defective Tax mutants K88A and V89A exhibited markedly reduced cytotoxic effects compared to the wild-type Tax protein.
Antigenicity of chimeric synthetic peptides based on HTLV-1 antigens and the impact of epitope orientation.
PMID: 11027594
2000
Biochemical and biophysical research communications
Abstract: The peptide M1 is a p19 (105-124) sequence, the peptide M2 is a gp46 (190-207) sequence, and the peptide M3 is a gp 46 sequence with substitution of proline at position 192 by serine.
In vivo analysis of human T-cell leukemia virus type 1 reverse transcription accuracy.
Abstract: Mutation of the methionine residue in the conserved YMDD motif of the HTLV-1 reverse transcriptase to either alanine or valine (i.e., M188A or M188V) led to a factor of two increase in the rate of mutation, indicating the role of this motif in enzyme accuracy.
p300 and p300/cAMP-responsive element-binding protein associated factor interact with human T-cell lymphotropic virus type-1 Tax in a multi-histone acetyltransferase/activator-enhancer complex.
PMID: 10766811
2000
The Journal of biological chemistry
Abstract: The Tax mutants (K88A and V89A) defective for p300/CBP-binding and LTR trans-activation, retained their abilities to interact with P/CAF.
Abstract: The M47 mutant (L319R, L320S) protein, which has previously been shown to interact with p300/CBP, by contrast, failed to form complexes with P/CAF and is impaired in LTR trans-activation.
HTLV-1 tax oncoprotein represses the p53-mediated trans-activation function through coactivator CBP sequestration.
Abstract: However, Tax with a mutation in the coactivator CBP-binding site (K88A), which activates NF-kappaB but not the CREB pathway, could not repress the p53 trans-activation function.
Four A6-TCR/peptide/HLA-A2 structures that generate very different T cell signals are nearly identical.
Abstract: The three-dimensional structures of the three A6-TCR/peptide/HLA-A2 complexes are remarkably similar to each other and to the wild-type agonist complex, with minor adjustments at the interface to accommodate the peptide substitutions (P6A, V7R, and Y8A).
PCAF interacts with tax and stimulates tax transactivation in a histone acetyltransferase-independent manner.
PMID: 10567539
1999
Molecular and cellular biology
Abstract: Point mutations at Tax amino acid 318 (TaxS318A) or 319 to 320 (Tax M47), which have decreased or no activity on the HTLV-1 promoter, are defective for PCAF binding.
Amino acid changes at positions 173 and 187 in the human T-cell leukemia virus type 1 surface glycoprotein induce specific neutralizing antibodies.
Abstract: We thus identified two amino acid changes, I173-->V and A187-->T, that play an important role in the antigenicity of neutralizable epitopes located in this region of the surface envelope glycoprotein.
Analysis of potential phosphorylation sites in human T cell leukemia virus type 1 Tax.
Abstract: Analysis of selected Tax mutants for ability to trans-activate the cytomegalovirus promoter demonstrated mutation of serine 77 to alanine reduced trans-activation by 90% compared to wild-type Tax.
HTLV1 mutation literature information.
PMID: 
2000
Immunity
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