Codon Usage Optimization and Construction of Plasmid Encoding Iranian Human Papillomavirus Type 16 E7 Oncogene for Lactococcus Lactis Subsp. Cremoris MG1363
PMID: 28441787
2017
Asian Pacific journal of cancer prevention
Abstract: In addition, onlyone common variant T234G was identified from all specimens, but it did not lead to any amino acid change.
Abstract: Themost frequently observed variant was C196T in E7 which led to an amino acid change of R66W.
Abstract: We alsodetected nucleotide variations A86G, and C188T in samples.
Discussion: All of Iranian cervical cancer showed variation T234G in the form of silent mutation, thus having no effect on protein.
Discussion: Also, the amino acid substitutions S63F (57.66 %), and R66W (72.39 %) were positioned at the C-terminal domain of the E7
E6 and E7 Gene Polymorphisms in Human Papillomavirus Types-58 and 33 Identified in Southwest China.
Result: K93N and R145 (I/N) of HPV-33 and HPV-58 E6 were observed at the same two positions.|
Result: In addition, there were no non-synonymous mutations in the HPV-58 E6 sequences encoding the alpha helix or the beta sheet; while three non-synonymous mutations (T74A, D76E, and V77A) occurred in HPV-58 E7 sequences encoding the alpha helix.
Result: In addition, two biallelic mutations and one triallelic (C706A/T) mutation were found over the 294-bp E7 ORF, resulting in 4 amino acid changes of S29T, A45V, A45E, and Q97L (Table 1).
A naturally occurring variant of HPV-16 E7 exerts increased transforming activity through acquisition of an additional phospho-acceptor site.
Abstract: A common E7 variant encodes an amino acid change at N29S.
Abstract: Biologically, these biochemical differences are reflected in an increased ability of the N29S variant to transform primary rodent cells.
Abstract: This is the first study to demonstrate an important biochemical change in E7 function caused by a naturally occurring variation, and we suggest that the N29S variant merits further assessment to determine whether it has an increased association with the development of HPV-associated malignancies.
HPV16 E6 polymorphism and physical state of viral genome in relation to the risk of cervical cancer in women from the south of Poland.
Abstract: For the first time, missense mutations G122A, C153T and G188A were discovered in EUR-350G variant.
The Cellular DNA Helicase ChlR1 Regulates Chromatin and Nuclear Matrix Attachment of the Human Papillomavirus 16 E2 Protein and High-Copy-Number Viral Genome Establishment.
Result: Following on from our previous experiments showing that BPV1 E2 associates with ChlR1 and that mutation of tryptophan 130 to arginine (W130R) abrogates ChlR1 binding, we aimed to determine whether this interaction surface is conserved in the HPV16 E2 protein.
Result: This was somewhat surprising because the Y131A mutation is not within the known nuclear localization signals in E2.
Result: To determine the biological significa
Table: D124A
Table: D173A
Table: E118A
Table: H130R
Table: K177A
Table: Y131A
Identification of the impact on T- and B- cell epitopes of human papillomavirus type-16 E6 and E7 variant in Southwest China.
Abstract: E6 72-83 for HLA-A*02:01 and E6 74-84 for HLA-B*15:02 maybe are the new direct for therapeutic vaccine aimed at L83V variants.
Abstract: For the B-cell epitopes, three most potent epitopes for E6 were listed, and N29S lead the growth of score from 0.81 to 0.83.
Abstract: The mutations of D25E and L83V of E6 and N29S of E7 produce new epitopes, and the percentile values change with them.
Physical Status and Variant Analysis of Human Papillomavirus 16 in Women from Shanghai.
PMID: 25968097
2016
Gynecologic and obstetric investigation
Abstract: Amino acid changes of D25E and N29S were the most common variations across the genome.
Abstract: Furthermore, 10 novel variations were identified in this study, which led to the 3 amino acid changes of S23I in E2 and E244K and T269I in E2/E4.
Distribution of human papilloma virus type 16 E6/E7 gene mutation in cervical precancer or cancer: A case control study in Guizhou Province, China.
Abstract: D32E/M28V/L94P mutation was higher in the cervical cancer and precancer compared with the prototype.
Abstract: HPV-16 E6/E7 genetic variations, such as D32E/M28V/L94P, are more prevalent in cervical cancer or precancer than those in the controls.
Abstract: Mutations were simultaneously detected at the E6-D32E (T96A) and E7-M28V (A82G)/L94P ( PMID: 26732286
2016
Protein expression and purification
Abstract: Coexpressing the mono-site mutant HPV16 L1 L469A with GroEL/ES increased L1-p yield by ~7 fold compared with strains expressing the wild-type L1 gene.
Analysis of Sequence Variation and Risk Association of Human Papillomavirus 52 Variants Circulating in Korea.
Abstract: We also found that a lineage B-specific mutation K93R (A379G) was associated with an increased risk of cervical neoplasia.
Result: Lineage C also harbored lineage-specific variations (Table 3), of which an E6 nonsynonymous mutation (L83V, concurrent mutations of C348G and G350T) and five E7 nonsynonymous mutations, S52D (concurrent mutations of A706G and G707A), Y55D (T727G), H61Y (C733T), D64N (
ViMRT
HPV mutation literature information.
PMID: 
2017
Asian Pacific journal of cancer prevention
