Abstract: The nonconservative substitutions, such as K93R, K93E in E6, T37I, and D38N in E7, affected multiple hypothetical epitopes in the B cell.
Human Papillomavirus E7 Oncoprotein Promotes Proliferation and Migration through the Transcription Factor E2F1 in Cervical Cancer Cells.
PMID: 33155930
2021
Anti-cancer agents in medicinal chemistry
Abstract: CONCLUSION: The HPV58 E7 T20I variant did not promote malignant behaviors compared with wild-type HPV58 E7.
Abstract: However, the HPV58 E7 T20I variant did not promote malignant behaviors compared with wildtype HPV58 E7.
Abstract: OBJECTIVE: This study aimed to determine whether the HPV58 E7 T20I (C632T) variant promotes the malignant behavior of cervical cancer cells and the underlying mechanism of the HR-HPV E7 oncoprotein involved in the development of cervical cancer.
Phylogenomic Analysis of Human Papillomavirus Type 31 and Cervical Carcinogenesis: A Study of 2093 Viral Genomes.
Abstract: A viral genome-wide association analysis identified SNPs within the A/B clade associated with CIN3+, including H23Y (C626T) (odds ratio = 1.60, confidence intervals = 1.17-2.19) located in the pRb CR2 binding-site within the E7 oncogene.
Discussion: A functional study has shown that a mutant version of the HPV6 E7 with the H23Y SNP has a higher affinity pRb binding site than the HPV6 wild type, leading to transactivation of the host's cell cycle genes via the E2F transcription factor.
Discussion: We show that the H23Y (SNP at position 626) in E7, is more prevalent in the A2 and B2 sublineages and creates an identical 21-DLYCYE-26 pRb binding site to HPV16 E7, which suggests a shared carcinogenic c
CK2 Phosphorylation of Human Papillomavirus 16 E2 on Serine 23 Promotes Interaction with TopBP1 and Is Critical for E2 Interaction with Mitotic Chromatin and the Viral Life Cycle.
Figure: (A) U2OS-Vec (top panels), E2-WT (middle panels), and E2-S23A were stained with TopBP1 or E2 as indicated.
Figure: (C) The extracts from panel B were immunoprecipitated with TopBP1, and both E2-WT and E2-S23D co-IP with TopBP1 (lanes 2 and 3).
Figure: (D) The number of whorls observed in rafts from WT and S23A samples was determined in 10 independent images from each sample.
Figure: (E) Invasive keratinocytes were observed only in the S23A samples; an example is highlighted by a white arrow.
Figure: (E) Staining of U2OS E2-WT (top panels) and U2OS E2- PMID: 34631976
2021
Virusdisease
Abstract: C140 Stop codon mutation has caused early truncation of E6 in three sequences to produce the conformational structural change.
Abstract: L83V mutation was observed in 76.2% sequences followed by S71C seen in 28.6% sequences.
Abstract: Asian sequences showing higher frequency of L83V mutations and exclusive presence of S71C and C140 Stop codon mutations may be linked with higher oncogenicity.
Abstract: In contrast, E7 was relatively more conserved showing D4E (4.7%), G88R (23.8%), I93T (9.5%) and C94S (9.5%) mutations.
Abstract: Mutations of
Genetic variability and functional implication of HPV16 from cervical intraepithelial neoplasia in Shanghai women.
Abstract: 6329G>T, a potential binding site for TATA-binding protein, is the most common in LCR variants.
Abstract: Amino acid substitutions (D32N/E, E36Q, H85Y, and E120D in E6 and N29H/S and R77C in E7) were predicted to have an effect on conserved structural and functional residues, and five amino acid substitutions (H85Y, E36Q, I34L, and D32E in E6; R77C in E7) would potentially change the secondary structure.
Evidence for Missing Positive Results for Human Papilloma Virus 45 (HPV-45) and HPV-59 with the SPF10-DEIA-LiPA25 (Version 1) Platform Compared to Type-Specific Real-Time Quantitative PCR Assays and Impact on Vaccine Effectiveness Estimates.
PMID: 32907991
2020
Journal of clinical microbiology
Abstract: Sanger sequencing showed no phylogenetic distinction between SPF10-missed and SPF10-detected HPV-59 variants, but variants bearing the A6562G single-nucleotide polymorphism (SNP) in the SPF10 target region were more likely to be missed (P = 0.0392).
Table: A6562G
Table: T6
Discussion: A6562G is located 11 bases from the beginning of the SPF10 target region, possibly causing less efficient SPF10 primer binding.
Discussion: Although no phylogenetic distinction was found between L1 sequences of SPF10-detected and -missed HPV-59 variants, more missed HPV-59 variants carried the A6562G SNP.
Discussion: Likewise, GP5+/6+-impaired primer hybridization on HPV-52 was found due to a synonymous T6764C substitution.
Characterization of major capsid protein (L1) variants of Human papillomavirus type 16 by cervical neoplastic status in Indian women: Phylogenetic and functional analysis.
Abstract: Sixty-one SNPs were detected in L1 genes resulting in 20 nonsynonymous amino acid substitutions of which N56T, N92T, L158F, V178G, N181I, K236T, K443Q, K454T, and K475R are reported in Indian isolates for the first time.
Abstract: The substitutions N181T, T353P, and T389S were significantly associated with high-grade cervical disease.
Abstract: These substitutions, when mapped on three-dimensional structure, revealed that 11 and 4 substitutions are part of exp
A Phase II, Prospective, Randomized, Multicenter, Open-Label Study of GX-188E, an HPV DNA Vaccine, in Patients with Cervical Intraepithelial Neoplasia 3.
Abstract: The HPV sequence analysis revealed that the HPV type 16 E6/E7 variants D25E, V83L, and N29S were inversely associated with histopathologic regression at V8.
Human Papillomavirus 58 E7 T20I/G63S Variant Isolated from an East Asian Population Possesses High Oncogenicity.
Abstract: Our previous international epidemiological studies revealed that HPV58 carrying an E7 natural variant, T20I/G63S (designated V1), was associated with a higher risk of cervical cancer.
Abstract: Since V1 is more commonly found in eastern Asia, our report provides insight into the design of HPV screening assays and selection of components for HPV vaccines in this region.IMPORTANCE Epidemiological studies have revealed that a wild-type variant of HPV58 carrying an E7 variation, T20I/G63S (V1), is associated with a higher risk of cervical cancer.
HPV mutation literature information.
PMID: 
2021
Journal of medical virology
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