Abstract: Significantly, an E6(R124G) mutant had reduced nuclear import activity, and the E6 deletion mutant lacking (121)KKQR(124) was not imported into the nucleus.
DNA replicative functions of highly-expressed, codon-optimized human papillomavirus proteins E1 and E2.
PMID: 12565157
2003
Journal of virological methods
Abstract: In contrast, however, an HPV 16 E2 mutant bearing an E39A substitution, reported previously to be severely compromised for DNA replication, was found to be reduced only two-fold in activity and, therefore, considered not sufficiently inactivated for use in vaccines that depend on endogenous protein expression.
Abstract: Under the assay conditions, the HPV 16 E1 mutant (W439R, G482D) did not support HPV origin-driven DNA synthesis.
Human papillomavirus type-16 variants in Quechua aboriginals from Argentina.
Abstract: We identified a new variant, the Argentine Quechua-51 (AQ-51), similar to B-14 plus two additional changes: G7842-->A and A7837-->C; phylogenetic inference allocated it in the Asian-American branch.
Association between human papillomavirus 16 E6 variants and human leukocyte antigen class I polymorphism in cervical cancer of Swedish women.
Abstract: Women with HLA-B*44, HLA-B*51, or HLA-B*57 who were infected with the HPV16 E6 variant L83V had an approximately four- to fivefold increased risk for cancer compared with controls (odds ratio [OR] = 3.5, 95% CI = 1.1-11.1, OR = 4.2, 95% CI = 1.19-14.69, or OR = 4.67, 95% CI = 1.2-18.6, respectively).
Mutational analysis of human papillomavirus type 16 major capsid protein L1: the cysteines affecting the intermolecular bonding and structure of L1-capsids.
Abstract: In the nonreducing SDS gel, where WT-capsids were separated into two bands of L1-trimers and L1-dimers, the C175S-trimer band was not detected, the C185S-dimer band was much less dense, and the C428S-trimer and C428S-dimer bands were not detected.
Abstract: Morphologically, the C175S, C185S, and C428S fractions appeared to consist mostly of heterogeneous rod-shaped tubules, of smaller spherical particles, and of only capsomeres, respectively, whereas C102S, C229S, and C379S resembled WT.
Abstract: Mutants C175S (C at
Inhibition of human papillomavirus DNA replication by small molecule antagonists of the E1-E2 protein interaction.
PMID: 12730224
2003
The Journal of biological chemistry
Abstract: Functional evidence that the transactivation domain is the target of inhibition was obtained by swapping this domain between a sensitive (HPV11) and a resistant (cottontail rabbit papillomavirus) E2 type and by identifying an amino acid substitution, E100A, that increases inhibition by approximately 10-fold.
Co-mutation of HPV16 E6 and E7 genes in Thai squamous cervical carcinomas.
Abstract: A mutation from T to G at nucleotide 178, leading to a change from aspartate to glutamate (D25E), was the most common variation accounting for 70%.
Abstract: Interestingly, 90% of these E6 (D25E) variants coincided with a specific type of E7 mutation, N29S.
Abstract: This is the first finding of coordinated change between E6 (D25E) and E7 (N29S) in HPV, which might indicate a specific variant for the Thai population.
Human papillomavirus type 16 E6 variants and HLA class II alleles among Japanese women with cervical cancer.
PMID: 12918070
2003
International journal of cancer
Abstract: Additionally, DRB1*1502 was positively associated with a particular E6 variant designated D25E (n = 25), although we could not find a significant association between HLA class II alleles and L83V variants (n = 16).
Activity of the human papillomavirus E6 PDZ-binding motif correlates with an enhanced morphological transformation of immortalized human keratinocytes.
Abstract: Changes to the epithelial phenotype of cells expressing a mutant E6 protein (Thr156Glu) that is unable to degrade discs large was significantly less marked, although they did show evidence of epithelial-mesenchymal transition.
Abstract: The altered phenotype was accentuated in cells expressing an E6 protein containing a mutation (Arg153Leu) within a protein kinase A recognition motif that abrogates protein kinase A's negative regulation of the activity of the PDZ-binding domain.
Variants of the long control region and the E6 oncogene in European human papillomavirus type 16 isolates: implications for cervical disease.
Abstract: In addition, we found a specific European E6 variant, L83V, to be enriched in high-grade lesions and cancer rather than a specific European long control region variant.
Result: E6-L83V, on the other hand, has previously been shown to be associated with high-grade lesions and cancer in Swedish women and Norwegian women (Zehbe et al, 1998b; Andrew Jenkins, personal communication).
Result: A statistically significant trend for an increased prevalence of E6-L83V with increasing severity of the lesion (P=0.04) was observed, while this was not the case for LCR-7193/7521 (P=0.15) (Table 2).
Result: In the Finnish group, E6-L83V was associated with progression
HPV mutation literature information.
PMID: 
2003
Journal of virology
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