Abstract: Mapping studies identified lysine 292 as the principal residue for covalent conjugation of SUMO to HPV16 E2, and a lysine 292 to arginine mutant showed defects for both transcriptional activation and repression.
Abstract: The expression levels, intracellular localization, and the DNA-binding activity of HPV16 E2 were unchanged by this K292R mutation, suggesting that the transcriptional defect reflects a functional contribution by sumoylation at this residue.
Result: Both WT and K292R mutant E2 proteins were predominantly nuclear and partly cytoplasmic, and there was no apparent difference in the distribution of these two types of 16E2 protein.
Result: but only the K292R mutant of 16E2 lost the ability to be sumoylated wh
Identification of an arginine-rich motif in human papillomavirus type 1 E1;E4 protein necessary for E4-mediated inhibition of cellular DNA synthesis in vitro and in cells.
Abstract: HPV1 E4-mediated replication inhibition in vitro and suppression of entry of HPV1 E4-expressing cells into S phase are both abrogated upon alanine replacement of arginine 45 in the full-length E4 protein (E1;E4), implying that these two HPV1 E4 functions are linked.
Human papillomavirus type 45 E7 is a transforming protein inducing retinoblastoma protein degradation and anchorage-independent cell cycle progression.
Abstract: HPV-45 E7C28G did not bind pRb and could neither induce pRb-proteolysis nor promote cell cycle progression.
Abstract: We demonstrate here that the HPV-45 E7 protein transforms immortalized NIH3T3 fibroblasts, while mutations in either the conserved LXCXE sequence (C28G) or the carboxyl-terminus (Delta87LQQLF91) significantly abolish this activity.
Distribution of HPV-16 intratypic variants among women with cervical intraepithelial neoplasia and invasive cervical cancer in Mongolia.
PMID: 19256739
2008
Asian Pacific journal of cancer prevention
Abstract: Among the remaining 31 variants, variants with the T350G nucleotide change were predominant (13/31, 42%), followed by variants containing G94A (11/31, 35%), G176A (4/31, 13%) and G274T (2/31, 7%).
Human Papillomavirus 58 E7 T20I/G63S Variant Isolated from an East Asian Population Possesses High Oncogenicity.
Abstract: Although this study has some limitations such as a small sample size and not enough clinical data, these finding suggests that the distinctive distribution of HPV 16 As-variant E6 D25E and E7 N29S might be associated with geographical dependence rather than disease progression.
Abstract: At the amino acid level, the isolates showed 14 variants including E6 Q14H, E6 D25E, E6 I27R, E6 H78Y, E6 L83V, and E7
[Association between human papillomavirus (HPV) type 16 infection and E6/E7 gene variant and the cervical lesions in Beijing].
PMID: 17429529
2007
Zhonghua shi yan he lin chuang bing du xue za zhi
Abstract: D32E/N29S mutation rate was 38.9% (28/72), the detection rate increased with the severity of cervical lesions (P < 0.05).
Abstract: A mutation, E6-D32E (T96G) coincided with a specific type of E7 mutation, N29S (A86G).
Abstract: CONCLUSION: HPV 16 was the most common type in women with cervical lesions in Beijing, D32E/N29S variant associated with the cervical lesions.
Activities of human papillomavirus 16 E6 natural variants in human keratinocytes.
Abstract: The I27R/L83V variant showed the lowest activity whereas the R8Q variant showed the highest activity.
Abstract: The L83V polymorphism previously associated with risk for cancer progression in some populations, showed significant activity, comparable to that of the E6 prototype, in reducing p53 and Bax levels.
Abstract: This, and augmentation of other described functions might result in differences in L83V pathogenicity.
An oligoarray for the detection of human papillomavirus type 16 variants.
PMID: 17877642
2007
International journal of gynecological cancer
Abstract: We applied a sensitive and specific stacking hybridization assay using an oligoarray for the detection of Asian-American (AA) and European (E) (E350G) HPV 16 variants.
HPV16 E6 natural variants exhibit different activities in functional assays relevant to the carcinogenic potential of E6.
Abstract: On the other hand, L83V displayed more efficient degradation of Bax and binding to E6BP, but lower binding to hDlg.
Abstract: The L83V polymorphism, previously associated with risk for cancer progression in some populations, expressed similar levels of activity as that of the E6 prototype in most functional assays.
Analysis of mutations in the E6/E7 oncogenes and L1 gene of human papillomavirus 16 cervical cancer isolates from China.
PMID: 16603519
2006
The Journal of general virology
Abstract: In addition to D25E and E113D, the following E6 variations were found in this study: R129K, E89Q, S138C, H78Y, L83V and F69L.
Abstract: Prevalences of HPV16 E6 D25E and E113D variants were 67.3 and 9 %, respectively.
Abstract: The following L1 variations were found in this study: S377A, K387E, E378D, K382E and T379P.
HPV mutation literature information.
PMID: 
2008
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