Virusliterature

HPV mutation literature information.

Human papillomavirus prevalence in invasive anal cancers in the United States before vaccine introduction.

PMID: 23609590 2013 Journal of lower genital tract disease

Abstract: Among HPV-16-positive cases, 37% were identified as prototype variant Ep, and 63% were nonprototypes: 33% Em, 12% E-G131G, 5% Af1, 4% AA/NA-1, 3% E-C109G, 3% E-G131T, 2% As, and 1% Af2.

Method: Subsequently, nucleotide identities at position 109, 131, 132, 143, 145, 178, 350 were determined with a Pyromark Q96 MD (Qiagen, Valencia, CA, USA) to identify the variants AA/NA-1, Af1, Af2, As, E-C109G, E-G131G, E-G350, Em, Ep.

Discussion: in anal neoplasia tissue from a mostly HIV positive population, which may support their hypothesis that infection with the E-G131G or E-G131T genotypes pose elevated risk for malignant progression.

Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11.

PMID: 23649705 2013 Medical microbiology and immunology

Abstract: A72E in E1 and Q86K in E2 proteins were exclusively present in a moderately aggressive disease, L1 alterations A476V and S486F were unique to a severe papillomatosis.

Abstract: HPV11s in both solitary papillomas had identical LCRs containing a T7546C polymorphism, which strongly attenuated LCR activity, as confirmed by site-directed mutagenesis.

Abstract: This strong attenuator polymorphism was also present in the other four genomes showing significantly higher activities, but in these o

Intratypic variants of human papillomavirus type 16 and risk of cervical neoplasia in Taiwan.

PMID: 23852681 2013 Journal of medical virology

Abstract: Similar significant associations with cervical intraepithelial neoplasia grade 3 or worse were also observed for distinct nucleotide substitutions, including T178A/G, A647G, A7730C/G, T7781C, G7842A, and C24T/G.

Molecular and evolutionary analysis of HPV16 E6 and E7 genes in Greek women.

PMID: 23946477 2013 Journal of medical microbiology

Abstract: Human papillomavirus type 16 (HPV16) non-European variants have been associated with persistent infection and cervical cancer development, while the L83V variant of the E6 gene has been correlated with the progression of cervical malignancy.

Abstract: Moreover, the prototype and E6 L83V variants were both prevalent in high- and low-grade malignancies in Greek women.

Abstract: Novel sequence variations were recorded within the E6 and E7 genes in cervical samples, characterized as (T350G) European variants.

Abstract: The E6

Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women.

PMID: 23953248 2013 BMC infectious diseases

Abstract: At the amino acid level, the most prevalent non-synonymous variants were L83V (T350G), H78Y (C335T), E113D (A442C), Q14D (C143G/G145T) and R10I (G132T), and were observed respectively in 65%, 41.8%, 38.8%, 30.1% and 23.3% of total samples.Moreover, HPV16 European variants were mostly identified in younger women at early clinical diagnosis stages.

Abstract: The Af and NA1 variants were detected in 31.1% and 11.6% of the HPV16 positive specimens, respectively, whereas, only 3% of cases were prototype E350T.

Result: However, in women over 45 years, two variant

Genetic variations of E6 and long control region of human papillomavirus type 16 from patients with cervical lesion in Liaoning, China.

PMID: 24099556 2013 BMC cancer

Conclusion: In summary, the finding of this study is that the R10G/L83V variations in E6 and C7294T co-variation in LCR was significantly associated with risk for developing >=CIN2,3.

Table: A7238C

Table: A7279T

Table: A7287C

Genetic variation of human papillomavirus type 16 in individual clinical specimens revealed by deep sequencing.

PMID: 24236186 2013 PloS one

Abstract: In transient replication assays, a novel E1 mutant found in ICC, E1 Q381E, showed reduced ability to support HPV16 origin-dependent replication.

Result: Further, nucleotide variation analyses showed a novel aa variation at position 381 from glutamine (prototype) to glutamic acid (Q381E) in sample 6.

Result: In contrast, Q381E exhibited a reduced activity for supporting HPV16 replication, and Y379F completely lost the replication activity as expected.

Result: Intriguingly, the nucleotide substitutions observed in the E1 gene of sample 6 cause amino-acid (aa) changes in the E1 protein: methionine at aa

Research on sequence variations analysis of HPV-16 type in Southwestern China.

PMID: 24601042 2013 European journal of gynaecological oncology

Abstract: RESULTS: Compared with the European-Germanyl31 (EG131), 20 mutations were detected, of which eight mutations were detected from all the four biopsies: 131(G-A)(Gly-Arg), 178 (T-G)(Asp-Glu),350 (G-T)(Val-Leu), 647 (A-G)(Asn-Asp),846 (T-C) (synonymous mutation), L1 966th (C-T)(synonymous mutation),L1 1302 (C-T)(synonymous mutation) and L1 1434th (A-G) (synonymous mutation).

Human papillomaviruses 53 and 66: clinical aspects and genetic analysis.

PMID: 22001139 2012 Virus research

Abstract: The L1 protein mutations S405P and D458N were exclusively found in patients with L-SILs.

Abstract: The novel L1 mutation P432L was statistically associated with L-SIL lesions (P=0.04) and its development reduced the L1 predicted antigenicity (up to -2.3 for Glu433).

The human papillomavirus type 16 E7 oncoprotein targets Myc-interacting zinc-finger protein-1.

PMID: 22099967 2012 Virology

Abstract: E7C24G retained E7-wild-type capability to inhibit Miz-1-dependent transactivation.

Abstract: The C-terminal E7Delta79LEDLL83-mutant with reduced Miz-1-binding capacity was impaired in its capability to repress p21(Cip1) expression; whereas the pRB-binding-deficient E7C24G-mutant inhibited p21(Cip1) expression similar to wild-type E7.

Result: E7C24G bound Miz-1 with wild-type efficiency.

Result: C33A cells were transiently transfected either with pX-HPV-16 E7 wild-type, pX-HPV-16 E7Delta79LEDLL83, pX-HPV-16 E7Delta52YNIVT56, pX-HPV-16 E7C24G or pX, as indicated.

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