Virusliterature

HPV mutation literature information.

Replication interference between human papillomavirus types 16 and 18 mediated by heterologous E1 helicases.

PMID: 24456830 2014 Virology journal

Abstract: HPV16 E1 co-precipitated with HPV18 E1 in the cell lysates, and an HPV16 E1 mutant Y379A, which bound to HPV18 E1 less efficiently, failed to inhibit HPV18 replication.

Method: The codon 379 of the E1 gene in pF16E1 was changed from TAC to GCA by using PCR to produce pF16E1-Y379A, which expres

Result: Furthermore, an amino-acid substitution mutant F16E1-Y379A, which is supposed to negate the ability of E1 to oligomerize on single-stranded DNA and to bind to the replication origin, did not inhibit HPV18 replication.

Result: Importantly, the OD mutant, F16E1-Y379A, showed reduced binding efficiency compared to F16E1.

Genetic variability of HPV-58 E6 and E7 genes in Southwest China.

PMID: 24368255 2014 Infection, genetics and evolution

Abstract: 4 single nucleotide changes were identified among the E6 sequences with 3/4 synonymous mutations (C187T, A260C, C307T) and 1/4 non-synonymous mutations (A388C, from Lys to Asn, in alpha helix).

Abstract: 8 single nucleotide changes were identified among the HPV-58 E7 sequences with 2/8 synonymous mutations (T726C, T744G) and 6/8 non-synonymous mutations (G599A, C632T, G694A, G760A, G761A, T803C).

Abstract: The most common mutations of E6 genes are

Functional effects of sequence variations in the E6 and E2 genes of human papillomavirus 16 European and Asian variants.

PMID: 24150786 2014 Journal of medical virology

Abstract: A rare variation, EUR E6-R10G, was found to shorten the half-life of p53 more efficiently than the other variations.

Abstract: By Western blot analysis, a similar ability to degrade p53 was observed among EUR E6, As E6, EUR E6-L83V and As E6-E113D.

Abstract: Furthermore, the site-directed mutagenesis revealed that E232K, which is a linked variation in the hinge region of As E2, was responsible for its enhanced repression ability.

Nucleotide polymorphisms of the human papillomavirus 16 E1 gene.

PMID: 23881083 2014 Archives of virology

Abstract: Of these nucleotide variations, A1668G, G2073A, T2169C, T2189C, A2453T, C2454T, A2587T and G2650A were identified only in high-grade dysplasia cases.

Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11.

PMID: 23649705 2013 Medical microbiology and immunology

Abstract: A72E in E1 and Q86K in E2 proteins were exclusively present in a moderately aggressive disease, L1 alterations A476V and S486F were unique to a severe papillomatosis.

Abstract: HPV11s in both solitary papillomas had identical LCRs containing a T7546C polymorphism, which strongly attenuated LCR activity, as confirmed by site-directed mutagenesis.

Abstract: This strong attenuator polymorphism was also present in the other four genomes showing significantly higher activities, but in these o

Research on sequence variations analysis of HPV-16 type in Southwestern China.

PMID: 24601042 2013 European journal of gynaecological oncology

Abstract: RESULTS: Compared with the European-Germanyl31 (EG131), 20 mutations were detected, of which eight mutations were detected from all the four biopsies: 131(G-A)(Gly-Arg), 178 (T-G)(Asp-Glu),350 (G-T)(Val-Leu), 647 (A-G)(Asn-Asp),846 (T-C) (synonymous mutation), L1 966th (C-T)(synonymous mutation),L1 1302 (C-T)(synonymous mutation) and L1 1434th (A-G) (synonymous mutation).

Genetic variation of human papillomavirus type 16 in individual clinical specimens revealed by deep sequencing.

PMID: 24236186 2013 PloS one

Abstract: In transient replication assays, a novel E1 mutant found in ICC, E1 Q381E, showed reduced ability to support HPV16 origin-dependent replication.

Result: Further, nucleotide variation analyses showed a novel aa variation at position 381 from glutamine (prototype) to glutamic acid (Q381E) in sample 6.

Result: In contrast, Q381E exhibited a reduced activity for supporting HPV16 replication, and Y379F completely lost the replication activity as expected.

Result: Intriguingly, the nucleotide substitutions observed in the E1 gene of sample 6 cause amino-acid (aa) changes in the E1 protein: methionine at aa

Genetic variations of E6 and long control region of human papillomavirus type 16 from patients with cervical lesion in Liaoning, China.

PMID: 24099556 2013 BMC cancer

Conclusion: In summary, the finding of this study is that the R10G/L83V variations in E6 and C7294T co-variation in LCR was significantly associated with risk for developing >=CIN2,3.

Table: A7238C

Table: A7279T

Table: A7287C

Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women.

PMID: 23953248 2013 BMC infectious diseases

Abstract: At the amino acid level, the most prevalent non-synonymous variants were L83V (T350G), H78Y (C335T), E113D (A442C), Q14D (C143G/G145T) and R10I (G132T), and were observed respectively in 65%, 41.8%, 38.8%, 30.1% and 23.3% of total samples.Moreover, HPV16 European variants were mostly identified in younger women at early clinical diagnosis stages.

Abstract: The Af and NA1 variants were detected in 31.1% and 11.6% of the HPV16 positive specimens, respectively, whereas, only 3% of cases were prototype E350T.

Result: However, in women over 45 years, two variant

Codon 72 polymorphism of p53 and HPV type 16 E6 variants as risk factors for patients with squamous epithelial lesion of the uterine cervix.

PMID: 23124863 2013 Journal of medical virology

Abstract: In addition, T350G HPV 16 variant was over-represented in p53 Arg homozygous women with cervical lesions.

Abstract: The T350G HPV 16 variant was the most frequent variant observed in the analyzed group of Italian women, showing a slight decreasing with the severity of the lesion.

Abstract: When p53 genotype and HPV 16 variants are considered together, no difference emerges between cases and controls so is not possible to assess that the oncogenic effect of HPV 16 T350G variant may be influenced by the p53 genotype.

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