Abstract: A rare variation, EUR E6-R10G, was found to shorten the half-life of p53 more efficiently than the other variations.
Abstract: By Western blot analysis, a similar ability to degrade p53 was observed among EUR E6, As E6, EUR E6-L83V and As E6-E113D.
Abstract: Furthermore, the site-directed mutagenesis revealed that E232K, which is a linked variation in the hinge region of As E2, was responsible for its enhanced repression ability.
Genetic variability of HPV-58 E6 and E7 genes in Southwest China.
PMID: 24368255
2014
Infection, genetics and evolution
Abstract: 4 single nucleotide changes were identified among the E6 sequences with 3/4 synonymous mutations (C187T, A260C, C307T) and 1/4 non-synonymous mutations (A388C, from Lys to Asn, in alpha helix).
Abstract: 8 single nucleotide changes were identified among the HPV-58 E7 sequences with 2/8 synonymous mutations (T726C, T744G) and 6/8 non-synonymous mutations (G599A, C632T, G694A, G760A, G761A, T803C).
Abstract: The most common mutations of E6 genes are
Replication interference between human papillomavirus types 16 and 18 mediated by heterologous E1 helicases.
Abstract: HPV16 E1 co-precipitated with HPV18 E1 in the cell lysates, and an HPV16 E1 mutant Y379A, which bound to HPV18 E1 less efficiently, failed to inhibit HPV18 replication.
Method: The codon 379 of the E1 gene in pF16E1 was changed from TAC to GCA by using PCR to produce pF16E1-Y379A, which expres
Result: Furthermore, an amino-acid substitution mutant F16E1-Y379A, which is supposed to negate the ability of E1 to oligomerize on single-stranded DNA and to bind to the replication origin, did not inhibit HPV18 replication.
Result: Importantly, the OD mutant, F16E1-Y379A, showed reduced binding efficiency compared to F16E1.
HPV16 E6 variants: frequency, association with HPV types and in silico analysis of the identified novel variants.
Abstract: Phylogenetic analysis revealed that the variant F69L + L83V is not related to any of these lineages, while the variant M137L + L83V is closely related to the North American variant.
Abstract: The results revealed that the European T350G was the most common variant (50%) followed by the European prototype (40.3%) and the North-American (N = 3; 4.8%).
Abstract: while the European T350G variants were seen in women with co-infections.
Restoration of MAGI-1 expression in human papillomavirus-positive tumor cells induces cell growth arrest and apoptosis.
Abstract: In this study, we describe the generation of a mutation, K499E, within the MAGI-1 PDZ1 domain, which is resistant to E6 targeting.
Abstract: While the K499E mutation does not significantly affect these intrinsic activities of MAGI-1 in HPV-negative cells, its resistance to E6 targeting in an HPV-positive setting results in more cells expressing the mutant MAGI-1 than the wild-type MAGI-1, with a corresponding increase in TJ assembly, induction of apoptosis, and reduction in cell proliferation.
Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF.
Abstract: Co-expression of a pRb-binding-deficient mutant (E7C24G) and p14ARF resulted in EC24G nucleolar accumulation, but not in a significant higher activation of rDNA transcription, suggesting that the inactivation of pRb is involved in this phenomenon.
HPV16 E6 and E6AP differentially cooperate to stimulate or augment Wnt signaling.
Abstract: Two new missense mutations in the E6 gene (C279G and A305C) were found (together or alone, in association with other mutations) in 44 of 124 cases.
A novel mutant of human papillomavirus type 18 E6E7 fusion gene and its transforming activity.
PMID: 25227848
2014
Asian Pacific journal of cancer prevention
Abstract: At the same time, for safety future clinical application, a mutant of HPV18 ofE6E7 fusion gene was generated by site-directed mutagenesis at L52G for the E6 protein and C98G for the E7 protein.
Roles for human papillomavirus type 16 l1 cysteine residues 161, 229, and 379 in genome encapsidation and capsid stability.
Discussion: C161S is the least affected mutant, but is still 3 times less stable than wild-type.
Discussion: C229S virions are non-infectious at either time point.
Discussion: In differentiating tissue, we found that the mutant genomes, C161S, C229S, and C379S, all produced lower viral titers at 10 and 20 days of tissue growth.
Discussion: It was also found that C161S and C379S virions are more than 50% less infectious than wild-type virus at both 10 and 20 days.
Discussion: The relative stabilities of C161S, C229S, and C379S virions show they are more fragile than wild-type.
HPV mutation literature information.
PMID: 
2014
Journal of medical virology
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