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 HPV mutation literature information.


  A prime/boost strategy using DNA/fowlpox recombinants expressing the genetically attenuated E6 protein as a putative vaccine against HPV-16-associated cancers.
 PMID: 25763880       2015       Journal of translational medicine
Abstract: METHODS: In this study, DNA and fowlpox virus recombinants expressing the E6F47R mutant of the HPV-16 E6 oncoprotein were generated, and their correct expression verified by RT-PCR, Western blotting and immunofluorescence.
Method: Briefly, after excision from the pX5-E6-F47R/6C6S plasmid using the EcoRI and NotI enzymes, the E6-F47R/6C6S gene was inserted into the pcDNA3 plasmid (Life Technologies Corp., Carlsbad, CA, USA).
Method: Briefly, the genetically mutated E6F47R gene of HPV-16 was amplified by PCR from the pcDNA3E6F47R plasmid and inserted downstream of the VVH6 vaccinia virus early/late promoter into the pFPMCS vector, which contained the 3-beta-hydroxy


  Identification of human papillomavirus-16 E6 variation in cervical cancer and their impact on T and B cell epitopes.
 PMID: 25800725       2015       Journal of virological methods
Abstract: Interestingly, the presence of variation H78Y and L83V result in creation of four new epitopes for the HLA-DQA1*0101/DQB1*0501.


  Association of human papillomavirus 16 E6 variants with cervical carcinoma and precursor lesions in women from Southern Mexico.
 PMID: 25889023       2015       Virology journal
Abstract: Eight new E6 variants were found and 2 of them lead to amino acid change: E-C183/G350 (I27T) and E-C306/G350 (K68T).
Introduction: Other polymorphisms including A131G, G132C, C143G, G145T, G176A, T178G and C335T generate the amino acid changes R10G/I, Q14H/D, D25E/N, I27R and H78Y, respectively.
Introduction: Several reports have shown the presence of common polymorphisms that generate amino acid changes in the


  Genetic variability in E6 and E7 oncogenes of human papillomavirus Type 16 from Congolese cervical cancer isolates.
 PMID: 25991921       2015       Infectious agents and cancer
Abstract: E7 genomic region was found to be highly conserved with two most common T789C and T795G (100%) silent variations.
Abstract: The missense mutation G622A (D21N) in the E7 region seems to be described for the first time in this study.
Abstract: The most frequently observed nucleotide variations were the missense C143G, G145T and C335T in E6 (100%), leading to the non-synonymous amino acid variation Q14D and H78Y.
Result: All African lines showed a common pattern of five characteristic mutations in E6, namely,


  Distribution of human papillomavirus type 16 variants in Lithuanian women with cervical cancer.
 PMID: 26739674       2015       Medicina (Kaunas, Lithuania)
Abstract: CONCLUSIONS: The European HPV 16 L83V variant is usually associated with high risk of cervical cancer among women.
Abstract: However, statistically significant difference was not achieved when comparing difference of L83V variants between investigated groups and in HPV 16 L83V variant and prototype distribution in CIN3/Ca in situ and cancer.
Abstract: In 33 cases (34.4%) HPV 16 prototype was detected; in 50 cases (52.1%), L83V variant; and in remaining 13 cases (13.5%), multivariant of HPV 16.
Abstract: The frequency of L83V variant in invasive cancer and carcinoma in situ samples was the same (66.7% and 62.0%, respectively; P=0.696).


  Human papillomavirus mediated inhibition of DNA damage sensing and repair drives skin carcinogenesis.
 PMID: 26511842       2015       Molecular cancer
Result: About 80 % of skin biopsies of K14-HPV8-E6K136N mice collected at 3, 5, 13 and 24 days after treatment showed a gammaH2AX staining intensity comparable to FVB/n
Figure: The K138N mutant completely abrogated the ability of HPV5-E6 to delay the repair of UVB induced T^T (n = 3 in duplicate; control vs.
Figure: a Paraffin embedded skin sections of UV treated skin from FVB/n-wt, K14-HPV8-E6wt and K14-HPV8-E6K136N mice were stained for T^T (magnification: 400x).
Figure: b Representative macroscopical images (upper panel) and histology (lower panel, magnification as indicated) of FVB/n-wt (n = 15), K14-HPV8-E6wt (n = 12) and K14-HPV8-E6K136N animals (n = 50) taken 24 days after UV-irradiation.


  Activities of E6 Protein of Human Papillomavirus 16 Asian Variant on miR-21 Up-regulation and Expression of Human Immune Response Genes.
 PMID: 25987069       2015       Asian Pacific journal of cancer prevention
Abstract: CONCLUSIONS: E6D25E of the HPV16As variant differed from the E6 prototype in its activities on epigenetic modulation and immune surveillance and this might be a key factor for the important role of this variant in cervical cancer progression.
Abstract: HCK1T cells expressing E6D25E or E6Pro were established by transducing retrovirus-containing E6D25E or 16E6Pro.
Abstract: Interestingly, E6D25E showed a higher activity of miR-21 induction than did E6Pro in C33A cells expressing E6 protein.
Abstract: Interestingly, when treated with HeLa-CM, IRFs 1, 3 and 7 as well as c


  The human papillomavirus 16 European-T350G E6 variant can immortalize but not transform keratinocytes in the absence of E7.
 PMID: 26318249       2015       Virology
Abstract: Of focus in this study is the European-T350G variant, which is characterized by an L>V amino acid substitution at residue 83 of the prototype E6 protein.
Abstract: To elucidate the functional effects of this polymorphism, we followed keratinocytes transduced with E-T350G E6 for over 60 passages and compared them to keratinocytes transduced, in parallel, with prototype or Asian-American (Q14H/L83V/H78Y) E6.
Abstract: We also found that E-T350G down-regulated E-cadherin compared to the other variants, providing a possible link between its population-based oncogenicity and host genetic variations.
Abstract: We found that although E-T350G 


  Variants of human papillomavirus type 16 predispose toward persistent infection.
 PMID: 26339417       2015       International journal of clinical and experimental pathology
Abstract: It was found that the variants T178G, T350G and A442C in the E6 gene, as well as C3158A and G3248A variants in the E2 gene were associated with persistent HPV16 infection.


  Human papillomavirus 16 oncoprotein E7 stimulates UBF1-mediated rDNA gene transcription, inhibiting a p53-independent activity of p14ARF.
 PMID: 24798431       2014       PloS one
Abstract: Co-expression of a pRb-binding-deficient mutant (E7C24G) and p14ARF resulted in EC24G nucleolar accumulation, but not in a significant higher activation of rDNA transcription, suggesting that the inactivation of pRb is involved in this phenomenon.



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