LCR based quick detection of hotspot G1896A mutation in patients with different spectrum of hepatitis B.
PMID: 33857724
2021
Journal of infection and public health
Abstract: G1896A switch is one of the hotspots in subjects affected with hepatitis B.
Abstract: Almost 29% (24/82) of the cases remarkably had the presence of G1896A mutation confirmed by LCR and direct sequencing.
Abstract: Polymerase chain reaction (PCR) and Nucleotide Sequencing was done to identify the G1896A mutation in the precore region of the genome.
Abstract: The precore G1896A mutation is responsible for one third of the patients suffering from precore stop codon mutation.
Abstract: The major purpose of the study was to screen G1986A mutations at a large scale and also to establish ligase chain reaction as a mutation testing tool.
Natural variability in surface antigen and reverse transcriptase domain of hepatitis B virus in treatment-naive chronic HBV-infected Egyptian patients.
Abstract: Additionally, 11 occult samples (19 %) were detected, in which the predominant mutations of HBsAg were S143L (7 samples) followed by D144A and T125M (4 samples each).
Abstract: Additionally, we detected viral quasispecies and revealed Y124H as a characteristic substitution in the RT domain for HBV isolates in Egypt.
Abstract: Eleven substitutions were found in the major hydrophilic region, including two novel ones (M103T and G130E) that were not correlated before with genotype D.
Abstract: The most predominant mutation was Y124H (47 samples, 82 %).
rt269I Type of Hepatitis B Virus (HBV) Polymerase versus rt269L Is More Prone to Mutations within HBV Genome in Chronic Patients Infected with Genotype C2: Evidence from Analysis of Full HBV Genotype C2 Genome.
Introduction: In particular, we showed that rt269I-type infection versus rt269L-type infection leads to enhanced mitochondrial stress-mediated type I interferon (IFN-I) production, resulting in HBV e antigen (HBeAg)-negative infections by generating preC mutations at 1896 (G to A).
Result: C-Q182K/* (C2444A/T or A2445G mutati
Result: A number of studies have reported that preC-W28Stop, a preC 1896 mutation, is significantly related to liver disease progression.
Hepatitis B virus precore/core region mutations and genotypes among hepatitis B virus chronic carriers in South-Eastern, Nigeria.
PMID: 33708042
2021
International journal of health sciences
Abstract: Other HBV precore region mutations that were detected include: G1899A, T1846A, G1862C, G1888A, T1821C, C1826T, A1827C, A1850T, C1858T, precore start codon Kozak sequence mutations and some novel core region mutations such as G/A1951T and G1957A.
Result: An assessment of the distribution of HBV precore/core region specific mutations among the HBV genotypes in Table 2 showed that there exists
Distinctive HBV Replication Capacity and Susceptibility to Tenofovir Induced by a Polymerase Point Mutation in Hepatoma Cell Lines and Primary Human Hepatocytes.
PMID: 33562603
2021
International journal of molecular sciences
Discussion: Additionally, as its region is not overlapped with surface gene either, the effect of the rtT301A mutation may be solely attributed to itself.
Discussion: As the next step, molecular modeling of RT mutants would be helpful to elucidate the role and mechanism of the rtT301A mutation in viral fitness.
Discussion: Discovery of the rtT301A mutation at the nadir point of HBV DNA during TDF treatment may have captured the evolution process of the tenofovir-resistant virus.
Discussion: Further investigation is required to know whether endogenous cofactors are possibly associated with HBV polymerase, and if so, whether their properties are altered by
Transcriptome analysis of hepatoma cells transfected with Basal Core Promoter (BCP) and Pre-Core (PC) mutant hepatitis B virus full genome construct.
PMID: 33595430
2021
The Journal of general virology
Abstract: Infections with Basal Core Promoter (BCP) (A1762T/G1764A) and Pre-Core (PC) (G1896A) hepatitis B virus HBeAg mutants are associated with severe liver injury.
Occult and active hepatitis B virus detection in donated blood in Sao Paulo, Brazil.
Discussion: Also, the HIV infection may influence the association between rtA194T mutation and TDF resistance.
Discussion: Apart from rtL180M, rtM204V and rtA194T mutations, rtM187V and rtV207L were also detected.
Discussion: But these results do not agree with the previous study that a clear association between rtA194T and viral load rebound reported by Sheldon.
Abstract: A genotype D-specific pattern of mutations (A12S/P33S/P46S/T36D-G) was identified in CI (median frequency, 81.7%), which determined a reduction in HBV DNA release of up to 1.5 log in vitro.
Result: Finally, in the presence of the quadruple mutant A12S/P33S/P46S/T36D, there was a 25.6% reduction in HBV DNA (4[3.7-4.1] logIU/mL), which accounted for a 1.4-log drop in HBV viremia relative to the wt virus (p = 0.00012).
Result: Furthermore, a reduction of 18.5% (4.3 [4.1-4.6] logIU/mL) occurred in the presence of the quadruple mutant A12S/P33S/P46S/T36G (p = 0.047
Rapid Turnover of Hepatitis B Virus Covalently Closed Circular DNA Indicated by Monitoring Emergence and Reversion of Signature-Mutation in Treated Chronic Hepatitis B Patients.
Abstract: APPROACH AND RESULTS: In this study, we retrospectively monitored the emergence and reversion of the rtM204I/V mutant, a signature lamivudine resistance (LAMR ) mutation serving as a biomarker of cccDNA turnover in liver biopsies and longitudinal serum samples from two clinical trials.
Discussion: Last, patients with rtM204I/V develop VB with relatively high viral loads, which also facilitate the amplification and sequencing of LAMR in serum samples.
Discussion: Second, the development or reversion of the rtM204I/V mutant in serum can occur rapidly (within several months) with continuation or withdrawal of antiviral therapy.
HBV mutation literature information.
PMID: 
2021
Journal of infection and public health
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