Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection.
Abstract: In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera.
Discussion: More importantly, the zero cross-reactivity to HBcAg of the NTR-HBeAg assay was demonstrated by systematic testing of cell culture samples and patient's sera of G1896A viral strains (Figure 4 and Figure 6).
Discussion: Moreover, several previous studies found some serum samples from patients who were infected by G1896A HBeAg-minus HBV strains were positive in commercial HBeAg assays, suggesting possible false-p
Increased hepatitis B virus quasispecies diversity is correlated with liver fibrosis progression.
PMID: 34029727
2021
Infection, genetics and evolution
Abstract: Specific mutations, such as A1762T, G1764A and G1896A, in the BCP/PC region were more common in patients with advanced liver disease and formed the majority of the viral quasispecies pool in patients with LC and HCC.
Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.
Abstract: Collectively, our results provide novel insights into the mechanism of ETV resistance of HBV RT caused by L180M and M204V mutations.
Abstract: Crystallography of HIV RTY115F/F116Y/Q151M/F160M/M184V, mimicking HBV RT L180M/M204V, showed that the F115 bulge (F88 in HBV RT) caused by the F160M mutation induced deviated binding of dCTP from its normal tight binding position.
Abstract: ETV-triphosphate (ETV-TP) exhibited competitive inhibition with dGTP in both wild-type (wt) RT and M204V RT, as observed using Lineweaver-Burk plots.
Molecular characterization of hepatitis B virus basal core promoter and precore region of isolates from chronic hepatitis B patients.
PMID: 34111075
2021
JPMA. The Journal of the Pakistan Medical Association
Abstract: Precore stop codon mutation G1896A was detected in 19 (38%) isolates; 17(34%) among negative patients and 2(4%) in positive patients.
Abstract: A rare G1764T mutation was also detected in 6(12%) isolates.
Abstract: Classic A1762T/G1764A double mutation was noted in 15(30%) isolates.
Abstract: The CG1802-1803 mutation was detected in 47(94%) isolates, while all the 50(100%) isolates had T1858A.
Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.
Abstract: IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed.
Abstract: This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria.
Conclusion: The high prevalence of the sQ129H escape mutation detected here points out the need for better screening procedures for HBV-infected individuals in order to avoid the spread of viral strains evading vaccine protection and thus posing a serious threat to the successful elimination of HBV.
Result: In total, the sQ129H IEM was detected in 18.2% (n = 8/44) of the individuals with available sequence.
Result: Other mutations detected on the
Hepatitis B virus genetic heterogeneity and drug resistance among jaundiced patients at Coast General Teaching and Referral Hospital, Mombasa County, Kenya.
PMID: 34234632
2021
International journal of health sciences
Result: Mutation rtM204V conferring resistance to 3CT occurred predominantly at 26.7% in all the patients followed by rtL180M (20.0%) and rtV173L at 13.3%.
Result: On the other hand, 5 patients (11.1%) had rtM204V mutations with rtV173L and rtL180M secondary mutations [Table 2].
Result: Six patients (13.3%) had rtV173L multiple mutations at rtM204V with rtL1
Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.
Abstract: All the patients harbouring rtA194T or CYE/CYEI at baseline achieved viral suppression by week 96 after TDF or TAF treatment.
Abstract: Both the A194T and a quadruple mutation CYEI (S106C, H126Y, D134E and L269I) in hepatitis B virus (HBV) polymerase reverse transcriptase domain (pol/RT) are suggested to be associated with treatment failure with tenofovir disoproxil fumarate (TDF).
Abstract: In total, six out of 3886 (0.2%) patients carried the rtA194T mutation, while only 1 patient carried a triple CYE and 2 patie
Fast and Sensitive Real-Time PCR Detection of Major Antiviral-Drug Resistance Mutations in Chronic Hepatitis B Patients by Use of a Predesigned Panel of Locked-Nucleic-Acid TaqMan Probes.
PMID: 34319801
2021
Journal of clinical microbiology
Abstract: Multiple-point mt, including rtL180M-rtM204V- rtN238A and rtL180M-rtM204I, were identified in only two children, resulting in LMV-ADF resistance and reduced ETV susceptibility.
Abstract: Single-point mt for LMV and ADF resistance were detected in 57.7% and 54.1% of the child and adult samples, respectively, with rtV207M (children, 42.3%; adults, 36.7%) and rtN238T/A (children, 15.4%; adults, 16.3%) being the most frequent mt in these populations.
Abstract: We developed a novel
Case of hepatitis B virus reactivation after ibrutinib therapy in which the patient remained negative for hepatitis B surface antigens throughout the clinical course.
Abstract: Interestingly, the patient remained negative for HBsAg throughout the clinical course owing to triple HBsAg escape mutations: Q101K, M133L, and G145A.
Characterization of Antigen Escape Mutations in Chronic HBV-Infected Patients in Upper Egypt.
Discussion: Carman et al described the first vaccine escape mutation resulted from the substitution of a glycine (G) residue at position 145 by an arginine (A) residue (G145R).
Discussion: HBV variants with combinations of HBs Ag (G145R or P120T) and polymerase (L526M plus M550V) mutations showed increased HBV replication resulting in a severe clinical course in transplanted patients.
Discussion: In addition, this study shows the presence of different vaccine mutants circulating among Egyptian CHB patients, some mutants such as G145R/A, and D144E/A are recognized as the commonly known vaccine escape mutants
HBV mutation literature information.
PMID: 
2021
Emerging microbes & infections
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