Virusliterature

E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus.

PMID: 34867835 2021 Frontiers in microbiology

Abstract: E2G/A/V/D mutations could strongly affect extracellular and intracellular level of HBsAg (p < 0.05).

Abstract: E2 mutations (E2G/A/V/D) existed in 21.8% (26/119) of OBIs, while no E2 mutations were found in the control group.

Abstract: Furthermore, N-terminal signal peptides, with a typical cleavage site for peptidase at positions 27 and 28, were exclusively detected in S proteins with secretion-defective mutants (E2G/A).

Abstract: Meanwhile, for E2G/A mutations, the relative intracellular HBsAg (110.7-338.3% vs. extracellular) and its fluorescence intensity (1.5-2.4-fold vs. with genotype-matched pHBV1.3B/C) were significantly higher (p_< 0.05).

A nuanced role of the small loop of hepatitis B virus small envelope protein in virion morphogenesis and secretion.

PMID: 34852809 2021 Journal of biomedical science

Method: Proline substitution mutants W196P, M197P, and M198P, were engineered by introducing a proline into the envelope ORF of the pol-null replicon.

Method: Similarly, envelope mutations W196F, W196S and W196L were introduced into the pol-null replicon plasmid.

Method: Single small loop mutations, W196C, M197T, and M198P, were introduced individually into the envelope ORF in pCHT-9/3091.

Result: 3A and B), suggesting that core-

PMID: 34835134 2021 Viruses

Discussion: In our study, the massively glycosylated (W3S) and glycosylation-deficient (N146G) HBV replicated normally, but the virion seemed to be stacked in the cells.

Discussion: In this W3S mutant, eight mutations in the RT domain were found, some of which were associated with massive glycosylation and silent antigenicity, but none of these have been reported as drug-resistant mutants (Figure 1B).

Discussion: The results confirmed that the patterns of particle formation were similar between the wild type and W3S, and thus massive glycosylation itself did not affect HBV particle formation (Figure 3A,B), though more sample required to show the profile of W3S particles and secretion of W3S HBsAg, i.e., the ma

Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97.

PMID: 34834922 2021 Viruses

5Result: Interestingly, in the absence of TX100, the side chain densities of Leu-97 in chains A and D (Figure 6c, L97*) pointed towards the center of the spike similar as Phe-97 in ""conformation 1"" of wt HBc whereas the densities of Leu-97 in chains B and C pointed towards the side of the spike similar as Phe-97 in ""conformation 2"" of

Abstract: Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L).

Result: In contrast, the map of HBc-F97L (EMDB 4417 and all maps generated in this study (Table 1), (Figure 6) were reconstructed from HBc-CLP purifications without TX100 (and any other detergents) and did not show a pocket factor.

Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations.

PMID: 34755817 2021 Revista do Instituto de Medicina Tropical de Sao Paulo

Abstract: Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naive patients.

Result: In the group of patients undergoing treatment, strains of HBV with resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV17

Table: L180M

Table: M204V

AutoVEM2: A flexible automated tool to analyze candidate key mutations and epidemic trends for virus.

PMID: 34512928 2021 Computational and structural biotechnology journal

Result: 5 of the 7 sites were missense mutations, including 356S>A (T192G), 444S>P (T456C), 807D>V (A1546T), 10R>K (G2337A) on P gene, and 331A>V (C

Discussion: Among them, some mutations, such as D614G and N501Y of SARS-CoV-2, T350G of HBV, and C659T of HVP-16, have been proved to play an important role in the viruses, indicating the reliability and effectiveness of AutoVEM2.

Discussion: For HBV, the C659T mutation, which causes A331V mutation on S gene, is reported to be associated with increasing the efficiency of HBV replication.

Precore and Basal Core Promoter Hepatitis B Virus (HBV) Variants Are Present From a Young Age and Differ Across HBV Genotypes.

PMID: 32860463 2021 Hepatology (Baltimore, Md.)

Abstract: CONCLUSIONS: PC variants can be present in HBV genotype A and are usually associated with C1858T, which preserves the pregenome encapsidation sequence.

Abstract: Seventeen of 20 participants with genotype A and PC had a compensatory C1858T mutation.

Impacts of the Percentage of Basal Core Promoter Mutation on the Progression of Liver Fibrosis After Hepatitis B e Antigen Seroconversion.

PMID: 32860707 2021 The Journal of infectious diseases

Abstract: CONCLUSIONS: The percentage of A1762T/G1764A mutations after HBeAg seroconversion was associated with liver fibrosis.

Abstract: Hepatitis B e antigen seroconversion age is positively correlated with the percentages of A1762T/G1764A mutation at inflammatory phase before HBeAg seroconversion.

Abstract: RESULTS: We demonstrated that the percentages of A1762T/G1764A mutation are significantly higher in subjects with an LSM >7 kPa than in those with an LSM <=7 kPa after HBeAg seroconversion.

Abstract: Subjects who underwent interferon, entecavir, or tenofovir disoproxil

A DNA Nanoflower-Assisted Separation-Free Nucleic Acid Detection Platform with a Commercial Pregnancy Test Strip.

PMID: 34432346 2021 Angewandte Chemie (International ed. in English)

Abstract: It is able to correctly identify the unmutated and rtL180M genome types of HBV in clinical samples.

Abstract: This simple, separation-free platform is highly specific, as demonstrated with the detection of rtL180M, a single-nucleotide polymorphism observed in hepatitis B virus (HBV) associated with antiviral drug resistance.

Recombinant HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.

PMID: 34076480 2021 Journal of virology

Abstract:

Abstract: Collectively, our results provide novel insights into the mechanism of ETV resistance of HBV RT caused by L180M and M204V mutations.

Abstract: Crystallography of HIV RTY115F/F116Y/Q151M/F160M/M184V, mimicking HBV RT L180M/M204V, showed that the F115 bulge (F88 in HBV RT) caused by the F160M mutation induced deviated binding of dCTP from its normal tight binding position.

Abstract: ETV-triphosphate (ETV-TP) exhibited competitive inhibition with dGTP in both wild-type (wt) RT and M204V RT, as observed using Lineweaver-Burk plots.