Case of hepatitis B virus reactivation after ibrutinib therapy in which the patient remained negative for hepatitis B surface antigens throughout the clinical course.
Abstract: Interestingly, the patient remained negative for HBsAg throughout the clinical course owing to triple HBsAg escape mutations: Q101K, M133L, and G145A.
The Impact of HBV Quasispecies Features on Immune Status in HBsAg+/HBsAb+ Patients With HBV Genotype C Using Next-Generation Sequencing.
Abstract: Especially, mutations in antigenic epitopes, such as I126S in CHB and I126T in HCC, could impact the conformational structure and alter the antigenicity/immunogenicity of HBsAg.
E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus.
Figure: Compared with pHBV1.3B, the fluorescence density of cells transfected with pHBV1.3B-E2G and pHBV1.3B-E2A was significantly higher (p < 0.0001), while pHBV1.3B-E2V and pHBV1.3B-E2D was significantly lower (p < 0.05) (A).
Figure: Compared with pHBV1.3C, the fluorescence density of cells transfected with pHBV1.3C-E2A was significantly higher (p < 0.001).
Figure: No significant changing was found between pHBV1.3C-E2D and pHBV1.3C (B).
Figure: The positive fluorescent cells transfected with pHBV1.3C-E2G were too few to be included in the fluorescence intensity statistical analysis.
Figure: The staining of cells transfected with pHBV1.3B-E2V/D and pHBV1.3C-E2D wa
A nuanced role of the small loop of hepatitis B virus small envelope protein in virion morphogenesis and secretion.
Discussion: A pre-S1 envelope mutation A119F, changing an alanine (A) to a phenylalanine (F), can erase the immature secretion phenotype of the mutant I97L and successfully restore the wild-type-like selective export of the mature genome.
Discussion: As a side note, it is worth mentioning here that neither envelope W196L nor W196S could support HDV virion secretion.
Discussion: However, we were intrigued that the same polymerase mutant YIDD with a different envelope mutation W196L, displayed an even stronger signal of virion-associated DNA, at least in this in vitro HuH-7 cell culture setting.
Discussion: If CYL-II is not
Analysis of the Physicochemical Properties, Replication and Pathophysiology of a Massively Glycosylated Hepatitis B Virus HBsAg Escape Mutant.
Method: HBeAg ELISA (HBeAg ELISA kit; Bioneovan, Beijing, China) and preS1 ELISA (Anti-HB Pre-S1 Antigen Quantitative ELISA Kit, Beacle, Kyoto, Japan) were also conducted according to the manufacturer's instructions, since a commercial HBsAg ELISA could not detect W3S sAg.
Method: In the case of pHB-W3S, four 5 mL aliquots (i.e., 20 mL) were used and they were finally combined into one 500 microL TNE solution.
Method: The XbaI-SpeI fragments of the pHB-WT were replaced with the region corresponding to W3S
Method: The cells were then transfected with 1 microg of pHB-WT, pHB-W3S or pHB-W3S (N146G).
Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97.
5Result: Interestingly, in the absence of TX100, the side chain densities of Leu-97 in chains A and D (Figure 6c, L97*) pointed towards the center of the spike similar as Phe-97 in ""conformation 1"" of wt HBc whereas the densities of Leu-97 in chains B and C pointed towards the side of the spike similar as Phe-97 in ""conformation 2"" of
Abstract: Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L).
Result: In contrast, the map of HBc-F97L (EMDB 4417 and all maps generated in this study (Table 1), (Figure 6) were reconstructed from HBc-CLP purifications without TX100 (and any other detergents) and did not show a pocket factor.
Compartmentalized evolution of hepatitis B virus contributes differently to the prognosis of hepatocellular carcinoma.
Abstract: APOBECs-related HBV mutations, including G1764A, were more frequent in the sera than in the adjacent tissues.
Abstract: HBV QC and A1762T/G1764A in the sera and tumors have contrary prognostic effects in HCC.
Abstract: High-frequent A1762T/G1764A in the sera predicted an unfavorable RFS (P < 0.001), whereas, in the tumors, it predicted a favorable RFS (P = 0.035).
Specific determination of hepatitis B e antigen by antibodies targeting precore unique epitope facilitates clinical diagnosis and drug evaluation against hepatitis B virus infection.
Abstract: In contrast to widely used commercial assays, the NTR-HBeAg completely eliminated the cross-reactivity with secreted HBcAg from precore mutant (G1896A) virus in either cell culture or patient sera.
Discussion: More importantly, the zero cross-reactivity to HBcAg of the NTR-HBeAg assay was demonstrated by systematic testing of cell culture samples and patient's sera of G1896A viral strains (Figure 4 and Figure 6).
Discussion: Moreover, several previous studies found some serum samples from patients who were infected by G1896A HBeAg-minus HBV strains were positive in commercial HBeAg assays, suggesting possible false-p
Multiple drug-resistant HBV mutation may contribute to poor response of adefovir + entecavir in entecavir-resistant patients.
PMID: 33571155
2021
Journal of infection in developing countries
Abstract: RESULTS: ETV-resistant mutants were continuously detected in 10 of the 12 patients, and multidrug-resistant (MDR) mutants, including a novel strain (rtL180M+A181V+T184A+S202G+M204V), were detected in two patients.
Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China.
HBV mutation literature information.
PMID: 
2021
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