Abstract: Collectively, our results provide novel insights into the mechanism of ETV resistance of HBV RT caused by L180M and M204V mutations.
Abstract: Crystallography of HIV RTY115F/F116Y/Q151M/F160M/M184V, mimicking HBV RT L180M/M204V, showed that the F115 bulge (F88 in HBV RT) caused by the F160M mutation induced deviated binding of dCTP from its normal tight binding position.
Abstract: ETV-triphosphate (ETV-TP) exhibited competitive inhibition with dGTP in both wild-type (wt) RT and M204V RT, as observed using Lineweaver-Burk plots.
Molecular characterization of hepatitis B virus basal core promoter and precore region of isolates from chronic hepatitis B patients.
PMID: 34111075
2021
JPMA. The Journal of the Pakistan Medical Association
Abstract: Precore stop codon mutation G1896A was detected in 19 (38%) isolates; 17(34%) among negative patients and 2(4%) in positive patients.
Abstract: A rare G1764T mutation was also detected in 6(12%) isolates.
Abstract: Classic A1762T/G1764A double mutation was noted in 15(30%) isolates.
Abstract: The CG1802-1803 mutation was detected in 47(94%) isolates, while all the 50(100%) isolates had T1858A.
Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria.
Abstract: IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed.
Abstract: This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria.
Discussion: All sequences obtained in this study were classified as HBV genotype E, which is known to show a clear genotypic divergence from all genotypes within the a determinant, where escape mutations can occur, reported that the types of polymorphisms at positions associated with escape mutations observed in HBV vary from one genotype to another and that the most common of these polymorphisms found in genotype E are T116N, P120L/S, Q129H/R, M133I,
Characterization of Antigen Escape Mutations in Chronic HBV-Infected Patients in Upper Egypt.
Introduction: Confocal microscopy revealed that the L77R mutant HBsAg are largely accumulated within the ER and Golgi.
Introduction: In addition, a single amino acid substitution R79K near the end of the large loop also blocked HBV virion secretion, while the secretion of HBsAg subviral particles was normal.
Introduction: In our earlier studies, a naturally occurring S gene mutation L77R at the large loop CYL-I resulted in more than tenfold reduced HBV virion secretion, and 2.8-fold reduced HBsAg in the medium of transfected HuH-7 cells.
Introduction: This phenomenon can be rescued by another naturally occurring mutation W74L in the same large loop.
Introduction: Using a small loop mutant
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations.
PMID: 34755817
2021
Revista do Instituto de Medicina Tropical de Sao Paulo
Abstract: Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naive patients.
Result: In the group of patients undergoing treatment, strains of HBV with resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV17
Table: L180M
Table: M204V
Table: V173L
E2 Site Mutations in S Protein Strongly Affect Hepatitis B Surface Antigen Detection in the Occult Hepatitis B Virus.
Abstract: E2G/A/V/D mutations could strongly affect extracellular and intracellular level of HBsAg (p < 0.05).
Abstract: E2 mutations (E2G/A/V/D) existed in 21.8% (26/119) of OBIs, while no E2 mutations were found in the control group.
Abstract: Furthermore, N-terminal signal peptides, with a typical cleavage site for peptidase at positions 27 and 28, were exclusively detected in S proteins with secretion-defective mutants (E2G/A).
Abstract: Meanwhile, for E2G/A mutations, the relative intracellular HBsAg (110.7-338.3% vs. extracellular) and its fluorescence intensity (1.5-2.4-fold vs. with genotype-matched pHBV1.3B/C) were significantly higher (p_< 0.05).
Abstract: Notably, unlike
AutoVEM2: A flexible automated tool to analyze candidate key mutations and epidemic trends for virus.
PMID: 34512928
2021
Computational and structural biotechnology journal
Conclusion: Through the analysis of 11,088 HBV genomes and 1637 HPV-16 genomes, some valuable mutations, including the T350G of HBV and the C659T of HPV-16, were detected.
Result: 5 of the 7 sites were missense mutations, including 356S>A (T192G), 444S>P (T456C), 807D>V (A1546T), 10R>K (G2337A) on P gene, and 331A>V (C659T) on the S gene (Table 7).
Discussion: Among them, some mutations, such as D614G and N501Y of SARS-CoV-2, T350G of HBV, and C659T of HVP-16, have been proved to play an important role in the viruses, indicating the reliability and effectiveness
Analysis of the Physicochemical Properties, Replication and Pathophysiology of a Massively Glycosylated Hepatitis B Virus HBsAg Escape Mutant.
Discussion: In our study, the massively glycosylated (W3S) and glycosylation-deficient (N146G) HBV replicated normally, but the virion seemed to be stacked in the cells.
Discussion: In this W3S mutant, eight mutations in the RT domain were found, some of which were associated with massive glycosylation and silent antigenicity, but none of these have been reported as drug-resistant mutants (Figure 1B).
Discussion: The results confirmed that the patterns of particle formation were similar between the wild type and W3S, and thus massive glycosylation itself did not affect HBV particle formation (Figure 3A,B), though more sample required to show the profile of W3S particles and secretion of W3S HBsAg, i.e., the ma
Binding of a Pocket Factor to Hepatitis B Virus Capsids Changes the Rotamer Conformation of Phenylalanine 97.
5Result: Interestingly, in the absence of TX100, the side chain densities of Leu-97 in chains A and D (Figure 6c, L97*) pointed towards the center of the spike similar as Phe-97 in ""conformation 1"" of wt HBc whereas the densities of Leu-97 in chains B and C pointed towards the side of the spike similar as Phe-97 in ""conformation 2"" of
Abstract: Similar changes occur in mutants with low secretion phenotypes (P5T and L60V) and in a mutant with a pre-mature secretion phenotype (F97L).
Result: In contrast, the map of HBc-F97L (EMDB 4417 and all maps generated in this study (Table 1), (Figure 6) were reconstructed from HBc-CLP purifications without TX100 (and any other detergents) and did not show a pocket factor.
HBV mutation literature information.
PMID: 
2021
Journal of virology
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