Virusliterature

The Effects and Underlying Mechanisms of Hepatitis B Virus X Gene Mutants on the Development of Hepatocellular Carcinoma.

PMID: 35223517 2022 Frontiers in oncology

Introduction: HBV mutations, including A1762T/G1764A, C1653T, T1753V, and T1674G/C, in the core promoter (CP) region of the viral genome are typically the ones that increase the risk of HCC.

Introduction: Here, we investigated tumorigenic effects of combo HBx mutations (A1762T/G1764A, C1653T, T1753C, and T1674G), Ct-HBx, and wild-type (WT) HBx on HBsAg of the Wild-Type and the G145R Escape Mutant, included in the New Multivalent Vaccine against Hepatitis B Virus, Dramatically Differ in their Effects on Leukocytes from Healthy Donors In Vitro.

PMID: 35293188 2022 Voprosy virusologii

Abstract: CONCLUSION: ELISA-based detection of escape mutations S143L, D144E and G145R can be used for routine diagnostics, especially in the risk groups.

Abstract: The G145R mutation was recognized using ELISA kit in almost all cases.

Abstract: The kit specifically recognized the S143L substitution in contrast to the S143T variant.

Abstract: The presence of neighbor mutation D144E can be assumed due to it special serological fingerprint.

In vitro investigation of HBV clinical isolates from Chinese patients reveals that genotype C isolates possess higher infectivity than genotype B isolates.

PMID: 35314401 2022 Virologica Sinica

Abstract: Moreover, we identified a naturally occurring mutation sL21S in small hepatitis B surface protein, which markedly decreased the infectivity of HBV genotype C isolates, but not that of genotype B isolates.

Molecular characterization of hepatitis B virus (HBV) isolated from a pediatric case of acute lymphoid leukemia, with a delayed response to antiviral treatment: a case report.

PMID: 35361141 2022 BMC pediatrics

Table: A11T

Table: A1480G

Table: A1511G

Table: A2092T

Table: A47T

Table: A895G

Table: A928T

Table: C1060A

Table: C144T

Table: C324T

Table: D36G

Table: E64D

Table: G1461C

Table: G3039T

Table: G68W

Table: L100I

Table: L311I

Table:

PMID: 33462964 2021 Hepatology research

Abstract: A recent Korean report showed that two patients with viral breakthrough during treatment with TDF-containing regimens were found to carry five reverse transcriptase (rt) mutations ([rt]S106C[C], rtH126Y[Y], rtD134E[E], rtM204I/V, and rtL269I [I]), with the C, Y, E, and I mutations being associated with tenofovir resistance.

Abstract: Four mutations (rtS106C, rtD134N/S[N/S], rt

Multiple drug-resistant HBV mutation may contribute to poor response of adefovir + entecavir in entecavir-resistant patients.

PMID: 33571155 2021 Journal of infection in developing countries

Abstract: RESULTS: ETV-resistant mutants were continuously detected in 10 of the 12 patients, and multidrug-resistant (MDR) mutants, including a novel strain (rtL180M+A181V+T184A+S202G+M204V), were detected in two patients.

Distinctive HBV Replication Capacity and Susceptibility to Tenofovir Induced by a Polymerase Point Mutation in Hepatoma Cell Lines and Primary Human Hepatocytes.

PMID: 33562603 2021 International journal of molecular sciences

Discussion: Additionally, as its region is not overlapped with surface gene either, the effect of the rtT301A mutation may be solely attributed to itself.

Discussion: As the next step, molecular modeling of RT mutants would be helpful to elucidate the role and mechanism of the rtT301A mutation in viral fitness.

Discussion: Discovery of the rtT301A mutation at the nadir point of HBV DNA during TDF treatment may have captured the evolution process of the tenofovir-resistant virus.

Discussion: Further investigation is required to know whether endogenous cofactors are possibly associated with HBV polymerase, and if so, whether their properties are altered by

Molecular characteristics of HBV infection among blood donors tested HBsAg reactive in a single ELISA test in southern China.

PMID: 33468062 2021 BMC infectious diseases

Abstract: A high-frequency mutation, T1719G (93.3%), was detected in the BCP/PC region, which reduced the viral replication.

Abstract: Mutations were found in the S gene, including Y100C, Y103I, G145R, and L175S, which can affect the detection of HBsAg.

Result: As shown in Table 2, 45.5% (5/11, 45.5%) S regions in 11 HBV-B isolates had N40S mutations.

Result: In addition, immune escape mutants containing Q129H, T131I/T, G145R, and E164V we

Spontaneous reactivation of hepatitis B virus with a frameshift mutation in the precore region in an elderly hepatitis B virus carrier with lifestyle-related diseases.

PMID: 33959934 2021 Clinical journal of gastroenterology

Abstract: Her HBV genome was typed as subgenotype B1 and possessed a frameshift mutation due to an insertion of T after nucleotide (nt) 1817 and G to A mutations at nt 1896 and nt 1899 (G1896A/G1899A) in the precore region as well as serine to glutamine substitution of amino acid 21 in the core protein.

Role of core protein mutations in the development of occult HBV infection.

PMID: 33453326 2021 Journal of hepatology

Abstract: An analysis of the effect of Cp mutants on intracellular or extracellular viral protein production indicated that the W62R mutation in Cp had a critical impact on the reduction of HBcAg and HBeAg production during HBV replication, whereas P50H and/or S74G mutations played a limited role in influencing viral protein production invivo.

Abstract: CONCLUSIONS: W62R and its combination mutations in HBV Cp might massively affect HBcAg and HBeAg production during viral replication, which, in turn, might contribute to the occurrence of OBI.

Abstract: The <