Result: A closer examination of the Southern blot profiles of HBV replicative intermediates revealed reproducibly the lack or reduction of the full-length RC form in mutant I97L (highlighted by a red asterisk in.
Result: Again, as highlighted by a red asterisk, fully mature full-length RC form in mutant I97L appeared to be greatly diminished.
Result: Again, relative to WT HBV, we observed a nearly 2-fold-lower level of viral DNA of mutant I97L at 3 dpi with 30 mug of DNA per mouse.
Result: Again, we observed lower levels of intrahepatic viral DNA of mutant I97L, relative to the WT HBV, at 3 days, 1 week, and 2 weeks postinjection.
Result: As described in the introduction, we observed previously an immature secretion phenotype of the HBc variant I97L in the tissue
Comparison of pre-S1/S2 variations of hepatitis B virus between asymptomatic carriers and cirrhotic/hepatocellular carcinoma-affected individuals.
PMID: 31501793
2019
Clinical and experimental hepatology
Abstract: Conclusions: According to the results, point mutations such as L11Q, N37S, K38R and A49V, as well as certain deletions, may be associated with HBV infection outcome, among an HBV genotype D pure population.
Abstract: The rate of critical point mutations, including L11Q, N37S and K38R, was significantly higher in the ASC group, whereas the A49V substitution rate was significantly higher in the LC/HCC group (p < 0.05).
Discussion: An L11Q point mutation was also located in the hepatocyte binding site (p10-36 in
Occult HBV infection in Chinese blood donors: role of N-glycosylation mutations and amino acid substitutions in S protein transmembrane domains.
Result: However, mutation Q16R resulted in decreased HBsAg secretion compared to wt M88 (EC/IC ratio: 0.57 versus 1.2) whereas a significant increase in HBsAg secretion was observed in P38.II (EC/IC ratio: 31 versus 0.5).
Result: Introduction of the N146D/S/Y mutations in plasmid M88 and P38.II by SDM did not significantly alter the EC and IC HBsAg production pattern, although a slight reduction in HBsAg secretion was observed for P38.II mutants, as reflected by the decreased HBsAg EC/IC ratio compared to that of wt HBsAg (5-8 versus 17) (Figure 3).
Result: Mutants N146D/S/Y showed an expected non-glycosylated profile.
Result: Mutations
Clinical and Virological Aspects of HBV Reactivation: A Focus on Acute Liver Failure.
Abstract: A mutation in C-region of HBsAg (L216*), was associated with reduced HBsAg production and secretion.
Abstract: An HBsAg mutation (L216*) was found to be more frequent in ALF patients and was associated with reduced HBsAg production and secretion.
Method: A stop codon mutation was found more frequently in the ALF group (SHB L216*, see Table 3) and was selected for further analysis.
Method: In addition, two more variants were used as controls, the SHB D144E which could be found in both
Hepatitis B virus reactivation sustained by a hepatitis B virus surface antigen immune-escape mutant isolate in a patient who was hepatitis B core antibody positive during treatment with sofosbuvir and velpatasvir for hepatitis C virus infection: a case report.
PMID: 31542053
2019
Journal of medical case reports
Abstract: Sequencing analysis revealed the hepatitis B virus genotype D3 and the presence of two relevant immune-escape mutations (P120S and T126I) in the major hydrophilic region by analyzing the S region.
Conclusion: In contrast, by analyzing the S region, two relevant mutations, P120S and T126I, localized in the major hydrophilic region, an immune-active HBsAg domain, were found.
Discussion: In the first case, genome sequencing revealed a T118K mutation in the S region, but in contrast to our description, the HBV viremia occurred 5 months after the EOT with daclatasvir and asunaprevir.
Tenofovir is a more suitable treatment than entecavir for chronic hepatitis B patients carrying naturally occurring rtM204I mutations.
PMID: 31543688
2019
World journal of gastroenterology
Discussion: Although the mechanism is unclear, a possible reason for the significant association between spontaneous rtM204I variants and liver fibrosis might also be related to HBV genotype C.
Discussion: Among these variants, spontaneous rtM204I/V variants were detected in approximately 1.5% of NAs-native patients, and the rtM204I variant was the dominant type.
Discussion: Another finding of this study is that spontaneous rtM204I variants could affect antiviral responsiveness in treatment-naive patients when they are treated with NAs.
Discussion: But there is still controversy regarding prevalence of the naturally occurring
Prevalence of Hepatitis B Virus Infection in Shenzhen, China, 2015-2018.
Abstract: NAs resistant mutation occurrence patterns were multitudinous; single mutation patterns of rtM204I/V and rtL180M occurrences accounted for majority, followed by the combinational mutation pattern L180M + M204I/V.
Introducti
Introduction: A total of 8 HBV classical mutation sites are conventionally tested for HBV patients in most clinical labs, including M204I/V, L180M, T184A/F/I/L/S, L181T/V, M250I/L/V, M236T, S202G, and V207I.
Association between host TNF-alpha, TGF-beta1, p53 polymorphisms, HBV X gene mutation, HBV viral load and the progression of HBV-associated chronic liver disease in Indonesian patients.
Result: In addition to previously reported mutations, we also found five variants of the X gene located in the HBV functional region, including L37I, S43P, H86P/R, L98I and T105A, which have not been reported in previous studies and may be specific for Indonesian HBV.
Result: Multinomial regression analysis confirmed that K130M/V131I mutations were correlated with CLD progression (OR, 7.629; 95% CI, 1.578-36.884; Table V).
Result: The dominant mutations in the basal core promoter (BCP) were K130M/V131I, as found in 12.6% (11/87) of CLD patients.
High frequency of drug resistance mutations in the HBV genome in ART-experienced HIV-coinfected patients in southwestern Nigeria.
Abstract: Between compartments, core gene variants revealed Arg94Leu, Glu86Arg and Ser41Thr while X gene variants revealed Phe73Val, Ala44Val, Ser146Ala and Ser147Pro.
Abstract: In addition, a virus surface antigen mis-sense mutation resulting in M125T was detected in all the samples and could account for surface antigen negativity and occult HBV status.
Abstract: In tumor compartments per se, several mis-sense mutations were detected, notably the classic T1762A/A1764G mutation in the
ViMRT
HBV mutation literature information.
PMID: 
2019
Journal of virology
