Virusliterature

EBV mutation literature information.

Epstein-Barr Virus Fusion with Epithelial Cells Triggered by gB Is Restricted by a gL Glycosylation Site.

PMID: 28956769 2017 Journal of virology

Abstract: Our previous study found that the gL glycosylation mutant N69L/S71V had an epithelial cell-specific hyperfusogenic phenotype.

Abstract: The gL_N69L/S71V mutant had a large increase in epithelial cell fusion activity of up to 300% greater than that of wild-type gL starting at early time points.

Nasopharyngeal carcinoma risk prediction via salivary detection of host and Epstein-Barr virus genetic variants.

PMID: 29221111 2017 Oncotarget

Abstract: In this study, in southern China, where NPC is endemic, a single nucleotide polymorphism (SNP) in the EBV-encoded RPMS1 gene (locus 155391: G > A [G155391A]) and seven host SNPs (rs1412829, rs28421666, rs2860580, rs2894207, rs31489, rs6774494, and rs9510787) were confirmed to be significantly associated with NPC risk in 50 NPC cases versus 54 hospital-based controls with throat washing specimens and 1925 NPC cases versus 1947 hospital-based controls with buffy coat samples, respectively.

Method: The most striking finding was a significant association between a single nucleotide polymorphism (SNP) in the EBV-encoded RPMS1 gene (locus 155391: G

ERK2 phosphorylation of EBNA1 serine 383 residue is important for EBNA1-dependent transactivation.

PMID: 27009860 2016 Oncotarget

Abstract: In accordance, ERK2 was found to phosphorylate EBNA1 serine 383 in a reaction suppressed by H20 (a structural congener of the ERK inhibitor), U0126 (an inhibitor of MEK kinase), and mutations at substrate (S383A) or putative ERK docking sites.

Abstract: Wild-type (S383) and phosphomimetic (S383D) EBNA1 demonstrated comparable transactivation function, which was suppressed by H20 or U0126.

Method: Cytoplasmic and nuclear proteins from BJ-FE1 S383 WT, and S383A MT cells treated with DMSO and U0126 for 3 days were washed in 1 mL of ice cold PBS and then fractionated.

Method: Different EBNA1 constructs (387-641 WT, 379-~641 WT, or MTs of S383A, I528S,

Clonal deleted latent membrane protein 1 variants of Epstein-Barr virus are predominant in European extranodal NK/T lymphomas and disappear during successful treatment.

PMID: 27061907 2016 International journal of cancer

Abstract: These results suggest that del30 may be associated with poor prognosis NK/TL and that strain evolution could be used as a potential marker to monitor treatment.

Exosomal sorting of the viral oncoprotein LMP1 is restrained by TRAF2 association at signalling endosomes.

PMID: 25865256 2015 Journal of extracellular vesicles

Abstract: Notably, growth assays in soft agar revealed that oncogenic properties of the palmitoylation-deficient LMP1 mutant C78A were diminished compared to wild-type LMP1.

Method: HEK293 cells were transfected as described above with pGK2-LMP1wt or pGK2LMP1-C78A or pEGFP-N1.

Method: pGK2-LMP1-C78A was constructed by targeted mutagenesis of pGK2-LMP1wt, using the QuikChange Lightning Site-Directed Mutagenesis Kit protocol (Agilent, Santa Clara, CA, USA) and 5'-TTCAGAAGAGACCTTCTCGCTCCACTTGGAGCCCTTTG-3' as primer.

Result: Indeed, LMP1-C78A was seemingly equally efficient in recruiting TRAF2 when compared to wtLMP1, suggesting the mutant may still activate

RanBPM regulates Zta-mediated transcriptional activity in Epstein-Barr virus.

PMID: 25900136 2015 The Journal of general virology

Abstract: Interestingly, Z-K12R, a sumoylation-defective mutant of Zta, demonstrated transcriptional activation capabilities that were stronger than those of Zta and apparently unaffected by RanBPM modulation.

DNA Damage Signaling Is Induced in the Absence of Epstein-Barr Virus (EBV) Lytic DNA Replication and in Response to Expression of ZEBRA.

PMID: 25950714 2015 PloS one

Abstract: ZEBRA mutants deficient in DNA binding, Z(R183E) and Z(S186E), did not induce foci of pATM.

Method: After 8 h the transfection reagent was replaced with growth media and cells were incubated for another 24 hours.The lentivirus construct P3465V was kindly provided by Bill Sugden.

Method: PCR fragments were digested with BamH1 and EcoRI and ligated into the P3465V construct digested with BamH1 and EcoRI.

Method: The plasmids pHD1013/Z, pHD1013/Z(S186A), pHD1013/Z(S186E), pHD1013/Z(

Sequence analysis of Epstein-Barr virus (EBV) early genes BARF1 and BHRF1 in NK/T cell lymphoma from Northern China.

PMID: 26337172 2015 Virology journal

Abstract: It has been found that V29A strains, a BARF1 mutant subtype, showed higher prevalence in NPC, which may suggest the association between this variation and nasopharyngeal carcinoma (NPC).

Abstract: RESULTS: Two major subtypes of BARF1 gene, designated as B95-8 and V29A subtype, were identified.

Abstract: The V29A subtype had one consistent amino acid change at amino acid residue 29 (V A).

Introduction: V29A strains, a BARF1 mutant subtype, showed higher prevalence in NPC, which may suggest

A single nucleotide polymorphism in the Epstein-Barr virus genome is strongly associated with a high risk of nasopharyngeal carcinoma.

PMID: 26675171 2015 Chinese journal of cancer

Abstract: Moreover, the frequency of the SNP G155391A was associated with NPC incidence but was not associated with the incidences of other .

Abstract: RESULTS: Based on EBV-encoded RPMS1 variations, a single nucleotide polymorphism (SNP) in the EBV genome (locus 155391: G>A, named G155391A) was associated with NPC in 157 cases and 319 healthy controls from an NPC endemic region in South China [P < 0.001, odds ratio (OR) = 4.47, 95% confidence interval (CI) 2.71-7.37].

Abstract: The combined analysis in 1109 cases and 2052 controls revealed that the SNP G155391A was strongly associate

Epstein-barr virus latent membrane protein 1 polymorphism in nasopharyngeal carcinoma and other oral cavity tumors in Russia.

PMID: 24009107 2014 Journal of medical virology

Abstract: In particular, the G212S substitution in LMP1 isolates investigated was not observed at all.

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