Abstract: The newly identified EBV subtypes, which contains four Chinese-specific NPC-associated amino acid substitutions (BALF2 V317M, BNRF1 G696R, V1222I and RPMS1 D51E), showed a robust positive association with the risk of NPC in China (Odds Ratio = 4.80, 20.00, 18.24 and 32.00 for 1, 2, 3 and 4 substitutions, respectively, P trend <0.001).
Method: In the present study, a novel mutation (BNRF1 V1222I, loc5399) was validated via nested PCR and Sanger sequencing using mouthwashes from 108
The influence of human genetic variation on Epstein-Barr virus sequence diversity.
Abstract: Three polymorphic regions in the human genome were found to be associated with EBV variation: one at the amino acid level (BRLF1:p.Lys316Glu); and two at the gene level (burden testing of rare variants in BALF5 and BBRF1).
Result: 2B) and the EBV amino acid BRLF1:p.Lys316Glu.
Result: BRLF1:p.Lys316Glu and BRLF1:p.Glu377Ala are in moderate LD (r2 = 0.55) in our dataset.
Result: BRLF1:p.Lys316Glu has not been described previously, but variation at the nearby residue 377 (BRLF1: PMID: 33242451
2021
Experimental and molecular pathology
Abstract: Additionally, the AVFDRKSDAK (A1S/P and V2F/M/L) and YHLIVDTDSL (I4L and L10R/V/G/H) epitopes were associated with 5 patterns of amino acid changes in EBNA3B and EBNA-2, respectively.
Structural and Biophysical Investigation of the Key Hotspots on the Surface of Epstein-Barr Nuclear Antigen 1 Essential for DNA Recognition and Pathogenesis.
PMID: 34268333
2021
Frontiers in molecular biosciences
Result: In the case of Y518A, the structural compactness also remained haphazard.
Result: In the case of the R469A mutation, the complex experiences significant divergence from the initial structure.
Result: In the case of the R469A, the predicted DeltaG was reported to -5.784 kcal/mol, while the dAffinity was also predicted to be reduced (-1,009.21 kcal/mol).
Result: In the case of wild type peptide complex, the first three eigenvectors contributed 35% variance to the total observed motion, while in R49A, 45%, K514 43%, Y518A 39%, R521A 54% while R522A accounted for 48% variance in motion.
Result: Intriguingly the dAffinity for R521A and R522A was com
Characterization of a cancer-associated Epstein-Barr virus EBNA1 variant reveals a novel interaction with PLOD1 and PLOD3.
Abstract: Here we show that, while these mutations do not impact EBNA1 plasmid maintenance function, one of them (Thr85Ala) decreases transcriptional activation and results in a gain of function interaction with PLOD1 and PLOD3.
Epstein-Barr Virus gH/gL and Kaposi's Sarcoma-Associated Herpesvirus gH/gL Bind to Different Sites on EphA2 To Trigger Fusion.
Abstract: In addition, the mutations located in the large groove of EBV gH/gL (R152A and G49C) also have decreased binding with EphA2.
Abstract: To determine whether this glycosylation site may be the binding region for EphA2, we compared the EphA2 binding activity of EBV gH/gL and the EBV gH/gL-N69L/S71V mutant.
Abstract: We found that EBV gH/gL-N69L/S71V had higher binding affinity for EphA2, indicating that the EBV gL N-glycosylation site might be responsible
Integrated Pan-Cancer Map of EBV-Associated Neoplasms Reveals Functional Host-Virus Interactions.
Abstract: For example, one-third of patients with EBV+ NK/T-cell lymphoma carried two novel nonsense variants (Q322X, G342X) of LMP1 and both variant proteins failed to restrict viral reactivation, confirming loss of virostatic function.
Result: Importantly, nearly all EBV+ NPC and DLBCL samples, and one-third of NKTCL samples, had one or two frequent nonsense variations in the LMP1 protein, Q322X and G342X.
Conservation and polymorphism of EBV RPMS1 gene in EBV-associated tumors and healthy individuals from endemic and non-endemic nasopharyngeal carcinoma areas in China.
Abstract: However, few studies have investigated the single-nucleotide polymorphisms (SNPs) of RPMS1, and only one SNP site (g155391a) has been reported to be associated with nasopharyngeal carcinoma occurrence.
Sequence variations of Epstein-Barr virus-encoded BARF1 gene in nasopharyngeal carcinomas and healthy donors from southern and northern China.
Abstract: For Chinese isolates, the B95-8 type was dominant in both southern and northern China, but the isolates from southern China showed a higher frequency of the B95-8t165545c subtype than the isolates from northern China (76.0%, 38/50 NPC cases and 50.7%, 37/73 healthy donors vs 26.4%, 24/91 NPC cases and 7.6%, 6/79 healthy donors, P < .0001).
Abstract: For EBV genomes, the B95-8P subtype was dominant in northern China, Europe, America, and Australia, while V29A was dominant in Africa.
Abstract: Furthermore, the B95-8t165545c subtype was more frequent in NPC cases than healthy donors in both southern China (P = .005) and northern China (P = .001).
Abstract: Only 1 consistent coding change,
Replacing C189 in the bZIP domain of Zta with S, T, V, or A changes DNA binding specificity to four types of double-stranded DNA.
PMID: 29772230
2018
Biochemical and biophysical research communications
Abstract: Binding of Zta(C189S) and Zta(C189T) to DNA containing modified cytosines (DNA(5mC|C), DNA(5hmC|C), and DNA(5mCG)) was reduced compared to Zta.
Abstract: Substitution of cysteine 189 of Zta to serine (Zta(C189S)) results in a virus that is unable to execute the lytic cycle, which was attributed to a change in binding to methylated DNA sequences.
Abstract: To learn more about the role of this position in defining sequence-specific DNA binding, we mutated cysteine 189 to four other amino acids, producing Zta(C189S), Zta(C189T),
EBV mutation literature information.
PMID: 
2021
Virus evolution
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